Hi, Stripping sites are usually found in the more seedy areas of large cities ... Oh wait you meant ... nevermind :-) If you developed the reaction with DAB, you may be in a difficult position. DAB is a really strong reaction. You might try a Mallory bleach to remove it. 0.3% sulfuric acid in 5% potassium premanganate for 5 min followed by 5% oxalic acid until it is clear (usually a couple of minutes). Unfortunately, I am not sure what effect this will have on the epitopes you are looking for, but this is usually what is used to clean precipitated DAB from instruments it should work on the tissue too. If you use another chromogen such as AEC it is much easier (to many people's dismay) to remove this end product. Using alkaline phosphatase will be easy to remove the chromogens as well. It would also be very easy to do this with fluorescent tags too. Just remove the coverslip and dip the slide in ethanol and it will remove the colored end product. Then just start over for the new antigen.
Good luck, Amos On Fri, Oct 28, 2011 at 11:50 AM, <histonet-requ...@lists.utsouthwestern.edu > wrote: > Message: 2 > Date: Thu, 27 Oct 2011 10:30:40 -0700 > From: "Claire Weston" <cwes...@valasciences.com> > Subject: [Histonet] Stripping antibodies > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <002401cc94ce$2619ca60$724d5f20$@com> > Content-Type: text/plain; charset="us-ascii" > > Hi! > > > > Does anyone have experience in IHC stripping antibodies from a tissue > section and re-probing with different antibodies? What is the best way to > do that? If you could share your stripping protocol or experience I would > appreciate it - thanks! > > > > Claire > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet