Thanks for the help!
We actually tried antigen retrieval in Holy water in a pressure cooker for 10
minutes. I even brought in an old priest and a young priest to perform the
staining but no luck! Sorry, no vendor for the holy water - the old priest
makes his own.
I enjoyed the laugh! This project has been hell!
Thanks,
Julie
Message: 21
Date: Wed, 2 Nov 2011 08:46:28 -0500
From: "Beckham, Sharon" <s...@stowers.org>
Subject: RE: [Histonet] Luciferase IHC
To: "'O'Donnell, Bill'" <billodonn...@catholichealth.net>, Kim Donadio
<one_angel_sec...@yahoo.com>, "Randolph-Habecker, Julie"
<jhabe...@fhcrc.org>, "histonet@lists.utsouthwestern.edu"
<histonet@lists.utsouthwestern.edu>
Message-ID:
<2c40e43d1f7a56408c4463fd245dddf98aee2...@exchmb-02.stowers-institute.org>
Content-Type: text/plain; charset="iso-8859-1"
You know sometimes I wish these emails had a "LIKE" button on them. I found
this quite funny and wanted to "LIKE" it. Too much facebook for me!
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of O'Donnell, Bill
Sent: Wednesday, November 02, 2011 8:19 AM
To: Kim Donadio; Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Luciferase IHC
Actually, I have found that holy water reduces its sensitivity, greatly
diminishes its signal and will destroy its reactivity. (Listen closely and you
will hear the slide scream and growl and tell you no one likes you) (It's only
Wednesday, what'll Friday look like?)
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kim Donadio
Sent: Tuesday, November 01, 2011 7:01 PM
To: Randolph-Habecker, Julie; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Luciferase IHC
You have to soak it in holy water, then all H311 will break out,,,,
Sorry, I couldnt resist.
________________________________
From: "Randolph-Habecker, Julie" <jhabe...@fhcrc.org>
To: histonet@lists.utsouthwestern.edu
Sent: Tuesday, November 1, 2011 6:49 PM
Subject: [Histonet] Luciferase IHC
Has anyone had good results staining for luciferase in FFPE tissue? If so, what
antibody did you use.
Thanks!!
Julie
Julie Randolph-Habecker, Ph.D.
Director, Experimental Histopathology Shared Resources
Fred Hutchinson Cancer Research Center
1100 Fairview Ave N, DE-360
Seattle WA 98109-1024
Tel: 206-667-6119
Fax: 206-667-6845
jhabe...@fhcrc.org
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End of Histonet Digest, Vol 96, Issue 3
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