Hello Jesus! Try Sanderson's Rapid Bone Stain from Dorn and Hart Microedge (www.dornandhart.com). This is a metachromatic stain and should help you to penetrate the resin if heated at 60C and stained for 5-10 minutes depending upon section thickness. Rinse the slide in dH2O @ 60C and blot dry gently. Stain longer or shorter as necessary. Jack
> From: jesus.w....@gmail.com > Date: Fri, 4 Nov 2011 16:41:20 -0500 > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Formic Acid Recipe > > Dear all, > > I was having trouble staining Human embryonic palatal mesenchymal cells with > multi-stain solution. My PI told me to use formic acid on the samples so that > it could increase the permeability of the methyl methacrylate. I am not sure > if this is enough information, but the cells were loaded on hydroxyapatite > scaffolds. I have also tried aniline blue and villaneuva stain. Any > information on how I can make a solution of formic acid is appreciated along > with maybe other stains I could try using. Each sample was grinded to about > 50 microns. Thank you. > > Best Regards, > > Jesus W. Hernandez > Graduate Student > Department of Biomedical Engineering > University of Texas at San Antonio > jesus.w....@gmail.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
