The purpose of getting the paraffin up to 84C is to evaporate out the Isopropanol that is used instead of Xylene. This does not mean that the paraffin should be kept at that temperature when it is not being used for the infiltration step. It should be in an oven or a paraffin pot at a temperature no higher than 65C then allow the microwave to heat it up to 84C. This also depends on the type of microwave that you are using. If the unit has a vacuum system that is being used during the infiltration step then the temperature does not have reach 84C to evaporate out the IPA. Read your user manual that from the manufacture, it should help.
Donna Willis, HT/HTL (ASCP) Histology Lab Manager Baylor University Medical Center-Dallas ph. 214-820-2465 donna.wil...@baylorhealth.edu -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cheska mt Sent: Monday, November 07, 2011 1:38 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Quiestion? From: chesk...@hotmail.com To: histonet@lists.utsouthwestern.edu Subject: Date: Mon, 7 Nov 2011 11:32:09 -0800 Hello I am currently studying to become a histotech. I wanted to know why should the paraffin in the microwave processor be kept at 84 degrees. Thanks _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************** This e-mail may contain confidential and/or privileged information. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden and possibly a violation of federal or state law and regulations. Baylor Health Care System, its subsidiaries, and affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
