Sounds to me Definitely more likely to be the freezing process. Do you know their process?
Andrea On Oct 22, 2011, at 5:05 PM, histopath...@gmail.com wrote: > I never said these were muscles. These are tumors removed and frozen the day > before and sent to us on dry ice. We know not to use frost-free freezers! > > On , "Esparza, Sandra" <sespa...@seton.org> wrote: >> I have never put my muscles in a -20 we use -70 to -80 freezer and have > > >> not had a problem with artifact. Is your freezer frost free? > > > > > >> Sandra > > >> Sandra Esparza HT(ASCP)QIHC > > >> Lead Technologist > > >> Dell Children's Medical Center of Central Texas > > >> 512-324-0000 x87061 > > >> sespa...@seton.org > > > > > >> -----Original Message----- > > >> From: histonet-boun...@lists.utsouthwestern.edu > > >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > > >> histopath...@gmail.com > > >> Sent: Friday, October 21, 2011 8:44 AM > > >> To: Histonet@lists.utsouthwestern.edu > > >> Subject: [Histonet] ? on frozen tissue artifacts > > > > > >> I work in a reference lab and some of our frozen sections exhibit severe > > > > > >> freeze artifact (ice crystals). The client claims it's something we're > > >> doing and we say they are not freezing them properly. We receive the > > >> specimens on dry ice and store them in a -20C freezer until we section > > >> them > > >> (no more than 1-2 days later). We never allow the tissues to thaw. My > > >> question: Can freeze artifact occur AFTER properly snap-freezing tissue > > >> or > > >> does this artifact ONLY occur during the initial preezing procedure? > > >> _______________________________________________ > > >> Histonet mailing list > > >> Histonet@lists.utsouthwestern.edu > > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet