Hello everyone! I have a problem in processing rat paws to paraffin embedding. I need to process and cut the whole paw (except fingers). So, first I need to decalcify the paw and then process it to paraffin embedding. My problem is that when I try to cut the paw I can not get a good section because the tissue have a gum-like consistence (mushy). Do you know where is my problem? Dehydration, clearing, embedding? ** *Note*: I decalcify the paws in formic acid and aluminum chloride for 24h; Then I wash samples in phosphate buffer saline for 5 hours. After that I did the following manual processing (applying vacuum): 2x30min in 70% ethanol, 2x30min in 80% ethanol, 2x30min in 96% ethanol, 2x30min in absolute ethanol, 3x15 min in Xilene, 2x15min in paraffin and a final incubation of 30min in paraffin. Then, I proceed to paraffin ambedding and try to cut sections of 5um of thickness.
If anyone can help me I will be grateful. Thanks in advance, Cátia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
