I have had folding and bubbles occur periodically with PFA fixed, sucrose cryoprotected frozen brain tissue I receive from other labs. I streak a distilled water moistened brush across the slide then pick up the frozen section on the moistened area. It comes out flat and smooth every time. I let the section dry as usual, then store in -20°C or -80°C. For ISH I use a RNase zapped and DEPC rinsed brush and streak DEPC water across the slide with the moistened brush. I section the PFA fixed, sucrose cryoprotected tissue around -21°C. Researchers report good subsequent IHC and ISH results and bring more samples.
Donna J. Emge, ASCP-HT Mouse Histology and Phenotyping Laboratory Manager Northwestern University Olson Pavilion 8-333 710 North Fairbanks Court Chicago, IL 60611 d-e...@northwestern.edu<mailto:d-e...@northwestern.edu> 312-503-2679 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
