@15 Wrap tissue in moist, not too wet!!, (0.9% salt or PBS) gauze and store at 4 degrees C. No/hardly any harm done to morphology, antigenicity, RNA, DNA and so on. Tissue is tougher than you think...
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0027704 > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. nuclear fast red (Patsy Ruegg) > 2. RE: nuclear fast red (Elizabeth Chlipala) > 3. Histologist needed in Austin Texas (Esparza, Sandra) > 4. validation (Amos Brooks) > 5. autostainer vials (Thomas Pier) > 6. Diane Tokugawa/CA/KAIPERM is out of the office. > (diane.tokug...@kp.org) > 7. Question regarding cutting on microtome (Marcia Spencer) > 8. RE: Question regarding cutting on microtome (Britton, Josette C) > 9. Chameleon tissue lifting off (Thomas, Nancy) > 10. (no subject) (jthaw...@shorememorial.org) > 11. RE: (no subject) (Rathborne, Toni) > 12. RE: Chameleon tissue lifting off (WILLIAM DESALVO) > 13. Immunohistochemical staining of type IV collagen in Alport's > Syndrome (CHRISTIE GOWAN) > 14. Part-time Job (Maria T) > 15. fresh specimens after hours (Houston, Ronald) > 16. RE: fresh specimens after hours (joelle weaver) > 17. ASR FISH probes (Martin, Erin) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Tue, 29 Nov 2011 11:29:07 -0700 > From: "Patsy Ruegg" <pru...@ihctech.net> > Subject: [Histonet] nuclear fast red > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <94C3B2CA09434506BBEB3246C1D686EB@Patsyoffice> > Content-Type: text/plain; charset="us-ascii" > > Is my nuclear fast red powder dead or does it take a long time with heat > and > stirring to turn red? It is pretty old, and one vial says it should be > stored below 0 but has not been. > > > > Cheers, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email <mailto:pru...@ihctech.net> pru...@ihctech.net > web site <http://www.ihctech.net> www.ihctech.net > > > > > This email is confidential and intended solely for the use of the > Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that > is > privileged & confidential within the meaning of applicable law. > Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. > If > you are NOT the intended recipient please contact the sender and dispose > of > this e-mail as soon as possible. > > > > > > ------------------------------ > > Message: 2 > Date: Tue, 29 Nov 2011 13:22:21 -0700 > From: Elizabeth Chlipala <l...@premierlab.com> > Subject: RE: [Histonet] nuclear fast red > To: Patsy Ruegg <pru...@ihctech.net>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <14E2C6176416974295479C64A11CB9AE011380AD9C8D@SBS2K8.premierlab.local> > Content-Type: text/plain; charset="us-ascii" > > Patsy > > It does not go completely in solution we filter prior to staining > > Liz > > ________________________________________ > From: histonet-boun...@lists.utsouthwestern.edu > [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg > [pru...@ihctech.net] > Sent: Tuesday, November 29, 2011 11:29 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] nuclear fast red > > Is my nuclear fast red powder dead or does it take a long time with heat > and > stirring to turn red? It is pretty old, and one vial says it should be > stored below 0 but has not been. > > > > Cheers, > > Patsy > > > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech, LLC > Fitzsimmons BioScience Park > 12635 Montview Blvd. Suite 215 > Aurora, CO 80010 > P-720-859-4060 > F-720-859-4110 > wk email <mailto:pru...@ihctech.net> pru...@ihctech.net > web site <http://www.ihctech.net> www.ihctech.net > > > > > This email is confidential and intended solely for the use of the > Person(s) > ('the intended recipient') to whom it was addressed. Any views or opinions > presented are solely those of the author. It may contain information that > is > privileged & confidential within the meaning of applicable law. > Accordingly > any dissemination, distribution, copying, or other use of this message, or > any of its contents, by any person other than the intended recipient may > constitute a breach of civil or criminal law and is strictly prohibited. > If > you are NOT the intended recipient please contact the sender and dispose > of > this e-mail as soon as possible. > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 3 > Date: Tue, 29 Nov 2011 14:52:25 -0600 > From: "Esparza, Sandra" <sespa...@seton.org> > Subject: [Histonet] Histologist needed in Austin Texas > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > <3d79f47dc92b204f9e5d35c885dfc5cb041df...@ausex2vs1.seton.org> > Content-Type: text/plain; charset="us-ascii" > > Full Time/Day Shift position open for a HT/HTL (ASCP) registered > histologist, with 2+ years working experience. Requires strong > embedding and microtomy skills, knowledge of muscle enzymes, EM, and IHC > is preferred. Proficiency in these areas can be obtained on the job. We > are a highly Specialized Histology department with state of the art > equipment. This position is at a Trauma 1 children's hospital located in > beautiful progressive Austin Texas. If you are interested in working in > a team environment please go to Seton.net to apply online. > > > > > > Sandra Esparza HT(ASCP)QIHC > > sespa...@seton.org > > > > ------------------------------ > > Message: 4 > Date: Tue, 29 Nov 2011 16:24:28 -0500 > From: Amos Brooks <amosbro...@gmail.com> > Subject: [Histonet] validation > To: histonet@lists.utsouthwestern.edu, amber.mcken...@gastrodocs.net > Message-ID: > <cac95ki9+nvq4qlsjkaro9ntkehqldsos3jefcyl2yh_yb5y...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi, > I would say no, and if any inspector disagreed you would be well > within > your rights to give him a good cuff upside the head. The point in having a > negative mouse and rabbit is to run them at the same concentration as > whatever primary you are running. That would mean you would need to > validate it at every conceivable dilution you could ever have a primary > antibody at. Kinda silly if you think about it. Any good validation for a > primary antibody should include a proper isotype negative control anyway, > but that is a separate can o' worms. > > Amos > > > On Tue, Nov 29, 2011 at 1:01 PM, > <histonet-requ...@lists.utsouthwestern.edu>wrote: > >> Message: 5 >> Date: Mon, 28 Nov 2011 20:00:03 +0000 >> From: Amber McKenzie <amber.mcken...@gastrodocs.net> >> Subject: [Histonet] validation >> To: "histonet@lists.utsouthwestern.edu" >> <histonet@lists.utsouthwestern.edu> >> Message-ID: <5a33c952bb67f4468af1f36d739212bc064...@jerry.gia.com> >> Content-Type: text/plain; charset="us-ascii" >> >> Do you have to validate the neg mouse and rabbit? >> > > > ------------------------------ > > Message: 5 > Date: Tue, 29 Nov 2011 16:30:17 -0600 > From: "Thomas Pier" <t...@medicine.wisc.edu> > Subject: [Histonet] autostainer vials > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <4ed5089a020000df0000c...@gwmail.medicine.wisc.edu> > Content-Type: text/plain; charset=US-ASCII > > I'm using a Labvision Autostainer 360. I typically wash and reuse the > reagent vials. Does anybody out there have any idea how long the vials > will last doing this? Is there anything I'm not thinking of that I should > be concerned about? > > Tom > > > ------------------------------ > > Message: 6 > Date: Tue, 29 Nov 2011 15:02:52 -0800 > From: diane.tokug...@kp.org > Subject: [Histonet] Diane Tokugawa/CA/KAIPERM is out of the office. > To: histonet@lists.utsouthwestern.edu > Message-ID: > <of2e5018c0.87390985-on88257957.007e9af3-88257957.007e9...@kp.org> > Content-Type: text/plain; charset=US-ASCII > > > I will be out of the office starting 11/29/2011 and will not return until > 12/05/2011. > > Note: For Cytology issues, please call Molly at 8-421-5487, Eric at > 8-421-5405, or Wanda 8-421-5426 For Histology issues, please call Mario > at 8-421-4961, general histology lab 8-421- 5408, Kiran at 8-421-5404, or > Wanda at 8-421-5426. > > ------------------------------ > > Message: 7 > Date: Tue, 29 Nov 2011 22:49:10 -0500 > From: Marcia Spencer <marcia.spence...@gmail.com> > Subject: [Histonet] Question regarding cutting on microtome > To: histonet@lists.utsouthwestern.edu > Message-ID: > <cama_98yr-ihrpiwvubdcw2io98rxm7m6jchodgsbfvswe2t...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > I am wondering what causes a "ribbon" appearance in skin when viewed > under the microscope, usually seen in the epidermis, but occasionally > can be seen in the underlying tissue as well. I find this effect > happens only in a few, 4 or 5 slides out of 100, but, I don't know how > to correct it. I would describe it as the "old fashion hard candy > that was rippled like a ribbon could be. Under the scope, focusing up > and down demonstrates that the section is complete, just not on the > same plane. Any suggestions? > Thank you for your time > M. Spencer > > On 11/27/11, histonet-requ...@lists.utsouthwestern.edu > <histonet-requ...@lists.utsouthwestern.edu> wrote: >> Send Histonet mailing list submissions to >> histonet@lists.utsouthwestern.edu >> >> To subscribe or unsubscribe via the World Wide Web, visit >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> or, via email, send a message with subject or body 'help' to >> histonet-requ...@lists.utsouthwestern.edu >> >> You can reach the person managing the list at >> histonet-ow...@lists.utsouthwestern.edu >> >> When replying, please edit your Subject line so it is more specific >> than "Re: Contents of Histonet digest..." >> >> >> Today's Topics: >> >> 1. dako autostainer labeler (Patsy Ruegg) >> >> >> ---------------------------------------------------------------------- >> >> Message: 1 >> Date: Sun, 27 Nov 2011 08:55:58 -0700 >> From: "Patsy Ruegg" <pru...@ihctech.net> >> Subject: [Histonet] dako autostainer labeler >> To: <histonet@lists.utsouthwestern.edu> >> Message-ID: <B29772909A06463CB04824EF6F2F4687@prueggihctechlt> >> Content-Type: text/plain; charset="us-ascii" >> >> Greetings, >> >> >> >> I am looking for another Seymour labeler like the one used on the Dako >> Autostainer, does anyone have one sitting around not being used I could >> purchase from you? It needs to have the software disc so I can install >> it >> on a computer. >> >> >> >> Regards, >> >> >> >> Patsy >> >> >> >> Patsy Ruegg, HT(ASCP)QIHC >> >> IHCtech >> >> 12635 Montview Blvd. Ste.215 >> >> Aurora, CO 80045 >> >> 720-859-4060 >> >> fax 720-859-4110 >> >> www.ihctech.net >> >> www.ihcrg.org >> >> >> >> >> >> ------------------------------ >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> End of Histonet Digest, Vol 96, Issue 39 >> **************************************** >> > > > > ------------------------------ > > Message: 8 > Date: Wed, 30 Nov 2011 09:08:42 -0500 > From: "Britton, Josette C" <jcbrit...@cheshire-med.com> > Subject: RE: [Histonet] Question regarding cutting on microtome > To: "Marcia Spencer" <marcia.spence...@gmail.com>, > <histonet@lists.utsouthwestern.edu> > Message-ID: > <f644cd64b2313f43bb3d2496b454cdac038c2...@cmc-ex01.cheshire-med.com> > Content-Type: text/plain; charset="us-ascii" > > Your waterbath is not hot enough! > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Marcia > Spencer > Sent: Tuesday, November 29, 2011 10:49 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Question regarding cutting on microtome > > > > I am wondering what causes a "ribbon" appearance in skin when viewed > > under the microscope, usually seen in the epidermis, but occasionally > > can be seen in the underlying tissue as well. I find this effect > > happens only in a few, 4 or 5 slides out of 100, but, I don't know how > > to correct it. I would describe it as the "old fashion hard candy > > that was rippled like a ribbon could be. Under the scope, focusing up > > and down demonstrates that the section is complete, just not on the > > same plane. Any suggestions? > > Thank you for your time > > M. Spencer > > > > On 11/27/11, histonet-requ...@lists.utsouthwestern.edu > > <histonet-requ...@lists.utsouthwestern.edu> wrote: > >> Send Histonet mailing list submissions to > >> histonet@lists.utsouthwestern.edu > >> > >> To subscribe or unsubscribe via the World Wide Web, visit > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> or, via email, send a message with subject or body 'help' to > >> histonet-requ...@lists.utsouthwestern.edu > >> > >> You can reach the person managing the list at > >> histonet-ow...@lists.utsouthwestern.edu > >> > >> When replying, please edit your Subject line so it is more specific > >> than "Re: Contents of Histonet digest..." > >> > >> > >> Today's Topics: > >> > >> 1. dako autostainer labeler (Patsy Ruegg) > >> > >> > >> ---------------------------------------------------------------------- > >> > >> Message: 1 > >> Date: Sun, 27 Nov 2011 08:55:58 -0700 > >> From: "Patsy Ruegg" <pru...@ihctech.net> > >> Subject: [Histonet] dako autostainer labeler > >> To: <histonet@lists.utsouthwestern.edu> > >> Message-ID: <B29772909A06463CB04824EF6F2F4687@prueggihctechlt> > >> Content-Type: text/plain; charset="us-ascii" > >> > >> Greetings, > >> > >> > >> > >> I am looking for another Seymour labeler like the one used on the Dako > >> Autostainer, does anyone have one sitting around not being used I > could > >> purchase from you? It needs to have the software disc so I can > install it > >> on a computer. > >> > >> > >> > >> Regards, > >> > >> > >> > >> Patsy > >> > >> > >> > >> Patsy Ruegg, HT(ASCP)QIHC > >> > >> IHCtech > >> > >> 12635 Montview Blvd. Ste.215 > >> > >> Aurora, CO 80045 > >> > >> 720-859-4060 > >> > >> fax 720-859-4110 > >> > >> www.ihctech.net > >> > >> www.ihcrg.org > >> > >> > >> > >> > >> > >> ------------------------------ > >> > >> _______________________________________________ > >> Histonet mailing list > >> Histonet@lists.utsouthwestern.edu > >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >> > >> End of Histonet Digest, Vol 96, Issue 39 > >> **************************************** > >> > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Wed, 30 Nov 2011 09:00:49 -0600 > From: "Thomas, Nancy" <n...@stowers.org> > Subject: [Histonet] Chameleon tissue lifting off > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > > <2c40e43d1f7a56408c4463fd245dddf993990...@exchmb-02.stowers-institute.org> > > Content-Type: text/plain; charset="us-ascii" > > I would like to ask anyone who sections chameleon tissue what type of > slides they use. I was having too much lifting while using the superfrost > plus slides. It looked like slides coated with Haupt's solution were > highly recommended, so I tried that. It is so much better, but still > there is some lifting. If someone is successful with sectioning and > staining lizard tissue without lifting, please advise me on type of > slides, drying times, or anything that might help. > Thank you so much, > > Nancy Thomas > Stowers Institute for Medical Research > Kansas City, MO > > > ------------------------------ > > Message: 10 > Date: Wed, 30 Nov 2011 10:19:46 -0500 > From: jthaw...@shorememorial.org > Subject: [Histonet] (no subject) > To: histonet@lists.utsouthwestern.edu > Message-ID: > > <ofe19cefa6.cc2acfaa-on85257958.0053ece9-85257958.00543...@shoremedicalcenter.org> > > Content-Type: text/plain; charset=US-ASCII > > > Hello All, > > We are having a issue with our intercom system in the OR so the > pathologist > are having issues communicating frozen section diagnosis with the surgeon. > Does anyone in Histoland use a written form to send frozen section > diagnosis? Per CAP if it is verbal the pathologist must speak directly > with > the surgeon. Any suggestions would be much appreciated. > > > Jennifer Thawley HT, ASCP > Histology Supervisor > Shore Memorial Hospital > (609) 653-3940 > > > This transmittal from Shore Memorial Health System is for the sole use of > the intended recipient and may contain confidential and privileged > information. Any unauthorized review or use, including disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender and destroy all copies of the transmittal. > > > > > ------------------------------ > > Message: 11 > Date: Wed, 30 Nov 2011 15:56:19 +0000 > From: "Rathborne, Toni" <trathbo...@somerset-healthcare.com> > Subject: RE: [Histonet] (no subject) > To: "'jthaw...@shorememorial.org'" <jthaw...@shorememorial.org>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > > <3ad061fe740d464fac7bf6b5cfb7570711f62...@smcmail01.somerset-healthcare.com> > > Content-Type: text/plain; charset="us-ascii" > > We switched from intercoms to phones some years back. The phone is put on > speaker mode in the OR. I suppose problems can occur with the phone system > too, but we haven't experienced this. > Regarding the CAP part of the question, I would call them. Ask if you can > send a written diagnosis, which you are to receive back with the > physician's/designee's signature. You can then document the intercom > problems, and how you worked around it. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of > jthaw...@shorememorial.org > Sent: Wednesday, November 30, 2011 10:20 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] (no subject) > > > Hello All, > > We are having a issue with our intercom system in the OR so the > pathologist are having issues communicating frozen section diagnosis with > the surgeon. > Does anyone in Histoland use a written form to send frozen section > diagnosis? Per CAP if it is verbal the pathologist must speak directly > with the surgeon. Any suggestions would be much appreciated. > > > Jennifer Thawley HT, ASCP > Histology Supervisor > Shore Memorial Hospital > (609) 653-3940 > > > This transmittal from Shore Memorial Health System is for the sole use of > the intended recipient and may contain confidential and privileged > information. Any unauthorized review or use, including disclosure or > distribution is prohibited. If you are not the intended recipient, please > contact the sender and destroy all copies of the transmittal. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > CONFIDENTIALITY NOTICE > This message and any included attachments are from Somerset Medical Center > and are intended only for the addressee. The information contained in > this > message is confidential and may contain privileged, confidential, > proprietary and/or trade secret information entitled to protection and/or > exemption from disclosure under applicable law. Unauthorized forwarding, > printing, copying, distribution, or use of such information is strictly > prohibited and may be unlawful. If you are not the addressee, please > promptly delete this message and notify the sender of the delivery error > by e-mail or you may call Somerset Medical Center's computer Help Desk > at 908-685-2200, ext. 4050. > > Be sure to visit Somerset Medical Center's Web site - > www.somersetmedicalcenter.com - for the most up-to-date news, > event listings, health information and more. > > > > ------------------------------ > > Message: 12 > Date: Wed, 30 Nov 2011 09:29:37 -0700 > From: WILLIAM DESALVO <wdesalvo....@hotmail.com> > Subject: RE: [Histonet] Chameleon tissue lifting off > To: <n...@stowers.org>, histonet <histonet@lists.utsouthwestern.edu> > Message-ID: <bay151-w315a9bc9a8c926aae0076a91...@phx.gbl> > Content-Type: text/plain; charset="iso-8859-1" > > > I worked w/ animal tissue, not specifically lizard, several years ago and > we always used Poly-L-Lysine coated slides. I found the polycationic > nature of this molecule seemed to interact well w/ non-human species and > created a strong interaction with the anionic sites of animal tissue > sections. Poly-L-Lysine always produced strong adhesive properties. We > also dried the sections at room temperature for 24 hours as we found that > longer and slower worked best for us. > > William DeSalvo, B.S., HTL(ASCP) > > > >> From: n...@stowers.org >> To: histonet@lists.utsouthwestern.edu >> Date: Wed, 30 Nov 2011 09:00:49 -0600 >> Subject: [Histonet] Chameleon tissue lifting off >> >> I would like to ask anyone who sections chameleon tissue what type of >> slides they use. I was having too much lifting while using the >> superfrost plus slides. It looked like slides coated with Haupt's >> solution were highly recommended, so I tried that. It is so much better, >> but still there is some lifting. If someone is successful with >> sectioning and staining lizard tissue without lifting, please advise me >> on type of slides, drying times, or anything that might help. >> Thank you so much, >> >> Nancy Thomas >> Stowers Institute for Medical Research >> Kansas City, MO >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 13 > Date: Wed, 30 Nov 2011 16:51:18 +0000 > From: CHRISTIE GOWAN <christiego...@msn.com> > Subject: [Histonet] Immunohistochemical staining of type IV collagen > in Alport's Syndrome > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <snt122-w21f8c48a161e5469e7aab2ae...@phx.gbl> > Content-Type: text/plain; charset="iso-8859-1" > > > We are looking into the feasability of doing this test in-house. Is anyone > doing this test for kidney and if so, how did you run your validation? We > have some positive patients but they are all skin so I guess we will need > to purchase controls specifically for kidney. If anyone has any experience > with this I would appreciate your comments. Thanks. > Christie Gowan > UAB University Hospital > > ------------------------------ > > Message: 14 > Date: Wed, 30 Nov 2011 08:56:17 -0800 (PST) > From: Maria T <histology.hous...@yahoo.com> > Subject: [Histonet] Part-time Job > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <1322672177.64453.yahoomail...@web140502.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=utf-8 > > Needed a PRN for a small GI Lab in Houston⦠The position is one week a > month from 2-10pm depending on workload. > Position includes grossing BXâs, processing, embedding and cutting. > If interested in position please email your resume along with any > questions. > > Thanks > > > ------------------------------ > > Message: 15 > Date: Wed, 30 Nov 2011 17:10:12 +0000 > From: "Houston, Ronald" <ronald.hous...@nationwidechildrens.org> > Subject: [Histonet] fresh specimens after hours > To: 'histo net' <histonet@lists.utsouthwestern.edu> > Message-ID: > <e5d0cd2352e46545a0c0ebe308ccce53191...@l1perdwxmb01.childrensroot.net> > > Content-Type: text/plain; charset="us-ascii" > > for facilities that are NOT staffed 24/7, how are fresh specimens handled > after hours? > > Up till now, OR staff have called the pathologist on-call to determine how > best to handle the specimen, but that process has broken down on the OR > side of things. The OR Director does not want 2 different ways of dealing > with fresh specimens and they now want a process that will be the same > 24/7. > > As we will not be staffing the lab 24/7 on the odd chance a fresh specimen > might come, I am interested in what other facilities may be doing. > > Thanks > Ronnie > > > Ronnie Houston, MS HT(ASCP)QIHC > Anatomic Pathology Manager > ChildLab, a Division of Nationwide Children's Hospital > www.childlab.com > > 700 Children's Drive > Columbus, OH 43205 > (P) 614-722-5450 > (F) 614-722-2899 > ronald.hous...@nationwidechildrens.org<mailto:ronald.hous...@nationwidechildrens.org> > www.NationwideChildrens.org<http://www.nationwidechildrens.org/> > > "One person with passion is better than forty people merely interested." > ~ E.M. Forster > > > > ----------------------------------------- Confidentiality Notice: > The following mail message, including any attachments, is for the > sole use of the intended recipient(s) and may contain confidential > and privileged information. The recipient is responsible to > maintain the confidentiality of this information and to use the > information only for authorized purposes. If you are not the > intended recipient (or authorized to receive information for the > intended recipient), you are hereby notified that any review, use, > disclosure, distribution, copying, printing, or action taken in > reliance on the contents of this e-mail is strictly prohibited. If > you have received this communication in error, please notify us > immediately by reply e-mail and destroy all copies of the original > message. Thank you. > > ------------------------------ > > Message: 16 > Date: Wed, 30 Nov 2011 17:23:00 +0000 > From: joelle weaver <joellewea...@hotmail.com> > Subject: RE: [Histonet] fresh specimens after hours > To: <ronald.hous...@nationwidechildrens.org>, Histonet > <histonet@lists.utsouthwestern.edu> > Message-ID: <snt135-w13a766857351c3770279ad8...@phx.gbl> > Content-Type: text/plain; charset="iso-8859-1" > > > RonnieFrom my experience when working in labs that are not staffed 24/7 > with HT or grossing personnel, after hours fresh specimens are called to a > pathologist on call by the OR staff, who may then make the judgment to > instruct them on fixation needed, or come in for a FS or view the > specimen. Most places have used a pager or page # system with an on call > rotation. Sometimes a histology person has been on -call after hours too ( > if they usually do the FS, accessioning etc at the bench in lieu of PA) or > just be available certain hours if the pathologist needs them to assist > with FS, other preparations for send outs etc.I think this is pretty much > what you have suggested, and that seems to be in alignment with the > arrangments and expectations I have worked for these circumstances in a > few of my positions without 24 hr staff.Joelle > > Joelle Weaver MAOM, BA, (HTL) ASCP > > http://www.linkedin.com/in/joelleweaver > > > From: ronald.hous...@nationwidechildrens.org >> To: histonet@lists.utsouthwestern.edu >> Date: Wed, 30 Nov 2011 17:10:12 +0000 >> Subject: [Histonet] fresh specimens after hours >> >> for facilities that are NOT staffed 24/7, how are fresh specimens >> handled after hours? >> >> Up till now, OR staff have called the pathologist on-call to determine >> how best to handle the specimen, but that process has broken down on the >> OR side of things. The OR Director does not want 2 different ways of >> dealing with fresh specimens and they now want a process that will be >> the same 24/7. >> >> As we will not be staffing the lab 24/7 on the odd chance a fresh >> specimen might come, I am interested in what other facilities may be >> doing. >> >> Thanks >> Ronnie >> >> >> Ronnie Houston, MS HT(ASCP)QIHC >> Anatomic Pathology Manager >> ChildLab, a Division of Nationwide Children's Hospital >> www.childlab.com >> >> 700 Children's Drive >> Columbus, OH 43205 >> (P) 614-722-5450 >> (F) 614-722-2899 >> ronald.hous...@nationwidechildrens.org<mailto:ronald.hous...@nationwidechildrens.org> >> www.NationwideChildrens.org<http://www.nationwidechildrens.org/> >> >> "One person with passion is better than forty people merely interested." >> ~ E.M. Forster >> >> >> >> ----------------------------------------- Confidentiality Notice: >> The following mail message, including any attachments, is for the >> sole use of the intended recipient(s) and may contain confidential >> and privileged information. The recipient is responsible to >> maintain the confidentiality of this information and to use the >> information only for authorized purposes. If you are not the >> intended recipient (or authorized to receive information for the >> intended recipient), you are hereby notified that any review, use, >> disclosure, distribution, copying, printing, or action taken in >> reliance on the contents of this e-mail is strictly prohibited. If >> you have received this communication in error, please notify us >> immediately by reply e-mail and destroy all copies of the original >> message. Thank you. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 17 > Date: Wed, 30 Nov 2011 17:55:14 +0000 > From: "Martin, Erin" <erin.mar...@ucsf.edu> > Subject: [Histonet] ASR FISH probes > To: histonet <histonet@lists.utsouthwestern.edu> > Message-ID: <24b7b291cc88d04ab663958e77a1f59d013...@ex09.net.ucsf.edu> > Content-Type: text/plain; charset=iso-8859-1 > > Hi all, > > > > More questions on FISH. How does one go about validating an ASR probe on > FFPE tissue? I have no FISH experience and therefore am starting from > scratch. The specific probes that our pathologists are interested in are: > t(14;18), t(17;22) and t(2;5). > > > > Also, does anyone know what educational and training background is > required to be able to do such a workup? I know IVDs are no problems, I > looked at the CAP checklist for molecular and it seems that this is > usually done in cytogenetics and the tech needs to at least have a BS and > experience with molecular. > > > > Any help would be greatly appreciated! > > > > Thanks, > > Erin > > > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 96, Issue 42 > **************************************** > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet