Scott,
Thank you for your recommendation of the Superfrost ultra plus slides.  I have 
just contacted the company for a sample box.  I received a few other 
suggestions too, and will keep that info for possible future use.  I will keep 
your email address, as well and maybe I will be able to help you sometime.
Thanks to others who replied.

Nancy Thomas



From: Scott Parker [mailto:spar...@vt.edu]
Sent: Friday, December 02, 2011 12:55 PM
To: Thomas, Nancy
Subject: Re: [Histonet] Chameleon tissue lifting off

Hello Nancy,

I work exclusively with squamate uterine and placental tissue so I know the 
frustration you are experiencing. Because placental tissues that I work with 
are so thin, separation is always a concern especially when using typical heat 
mediated antigen-retrieval techniques for immunohistochemistry.

I actually have good success with Thermo Scientific Superfrost ultra plus 
slides. We have best success with 5 um sections and air drying slides slowly on 
a slide warmer. We then bake the slides for 30 minutes at 60 C. As usual, 
carefully sectioning is also the first step in achieving good tissue adherence. 
With delicate reptile placental tissues, any imperfections on the sectioning 
side will lead to greater tissue detachment. I hope this helps. We should stay 
in touch as there are not too many of us that work on reptile tissue in the 
histo-sphere.

Scott

Scott L. Parker, Ph.D.
Assistant Professor of Biology
Department of Biology
Coastal Carolina University
SC 29528-6054
On Wed, Nov 30, 2011 at 10:00 AM, Thomas, Nancy 
<n...@stowers.org<mailto:n...@stowers.org>> wrote:
I would like to ask anyone who sections chameleon tissue what type of slides 
they use.  I was having too much lifting while using the superfrost plus 
slides.  It looked like slides coated with Haupt's solution were highly 
recommended, so I tried that.  It is so much better, but still there is some 
lifting.  If someone is successful with sectioning and staining lizard tissue 
without lifting, please advise me on type of slides, drying times, or anything 
that might help.
Thank you so much,

Nancy Thomas
Stowers Institute for Medical Research
Kansas City, MO
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu>
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Reply via email to