Hi Everyone,
 
Does anyone know how close in proximity 2 proteins need to be for them to show 
up as co-localized via immunofluorescence staining?
 
I am trying to compare routine 2-color IF vs. the Proximity Ligation Assay 
(which will not show co-localization unless the proteins are withing 40 nM of 
each other).
 
Thanks,
Kim

Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Reply via email to