I would not use any freezing aerosols if there is any suspicion of infection in tissue or cryochamber has been used for prior sectioning without being decontaminated. Depending on how fatty the tissue is you need to be in the region -28 to -35 degs C on the specimen, knife and anti-roll plate, for that you need a decent cryostat.
Best regards Alan Bright Sent from my BlackBerry® wireless device -----Original Message----- From: Miha Tesar <sauco...@yahoo.com> Sender: histonet-boun...@lists.utsouthwestern.edu Date: Wed, 21 Dec 2011 23:07:26 To: naveeda arshad<naveedafa...@yahoo.ca>; histonet@lists.utsouthwestern.edu<histonet@lists.utsouthwestern.edu> Reply-To: Miha Tesar <sauco...@yahoo.com> Subject: Re: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 Haloha! Does any one has any idea about how to cut the fat tissue in cryostat (frozen section). Temperature of the chambre/specimen and the thickness of the slice. I use the cryo spray but does not help me much! THX Miha and Mary C. 2 all ________________________________ From: naveeda arshad <naveedafa...@yahoo.ca> To: histonet@lists.utsouthwestern.edu Sent: Thursday, December 22, 2011 4:19 AM Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 25 Hi Does make in house gram Positive and negative control in your lab. What kind of tissue is suitable and and i need detail procedure for that.Thanks --- On Wed, 12/21/11, histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> wrote: From: histonet-requ...@lists.utsouthwestern.edu <histonet-requ...@lists.utsouthwestern.edu> Subject: Histonet Digest, Vol 97, Issue 25 To: histonet@lists.utsouthwestern.edu Received: Wednesday, December 21, 2011, 10:17 PM Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. RE: Control Slides (Rene J Buesa) 2. Great Job Opportunity in San Fran Bay Area! (Heidi Hawthorne) ---------------------------------------------------------------------- Message: 1 Date: Wed, 21 Dec 2011 09:36:27 -0800 (PST) From: Rene J Buesa <rjbu...@yahoo.com> Subject: RE: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu, MargaretSherwood <msherw...@partners.org> Message-ID: <1324488987.20130.yahoomailclas...@web65716.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Check with a colleague working in a hospital and ask for fresh tissue (for IHC) or (+) pathological cases. René J. --- On Wed, 12/21/11, Sherwood, Margaret <msherw...@partners.org> wrote: From: Sherwood, Margaret <msherw...@partners.org> Subject: RE: [Histonet] Control Slides To: "Rene J Buesa" <rjbu...@yahoo.com>, histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 12:15 PM That is ideal Rene, but we are a research lab and don't run the same tissue all the time. Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org From: Rene J Buesa [mailto:rjbu...@yahoo.com] Sent: Wednesday, December 21, 2011 12:05 PM To: histonet@lists.utsouthwestern.edu; Sherwood, Margaret Subject: Re: [Histonet] Control Slides I never bought a single (+) control slide. I prepared mine from the (+) cases we had. I think you should try this avenue as well. René J. --- On Wed, 12/21/11, Sherwood, Margaret <msherw...@partners.org> wrote: From: Sherwood, Margaret <msherw...@partners.org> Subject: Re: [Histonet] Control Slides To: histonet@lists.utsouthwestern.edu Date: Wednesday, December 21, 2011, 11:52 AM To all: I know this has come up before, but where do most people buy their (+) control slides for special stains? Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 50 Blossom Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 2 Date: Wed, 21 Dec 2011 09:41:24 -0800 From: Heidi Hawthorne <heidi.hawtho...@onassignment.com> Subject: [Histonet] Great Job Opportunity in San Fran Bay Area! To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <26c4a3b38503bc4cbbf4d986c3bc9b832290d1f...@oasslcexm01.oaifield.onasgn.com> Content-Type: text/plain; charset="us-ascii" Hello, We have an immediate need for a Histotechnician for our client in the East Bay of San Francisco. Approximately 3 month assignment, day/early morning shift available working in a hospital lab. Requirements: At least 6 months of paid experience in a hospital histology laboratory preparing and mounting pathological tissue specimens. Email your resume today for immediate consideration! Heidi Hawthorne Sr. Account Executive On Assignment, Inc. t: (510) 663-8622 c: (510) 435-7326 f: (866) 741-0805 heidi.hawtho...@onassignment.com<mailto:heidi.hawtho...@onassignment.com> www.onassignment.com<http://www.onassignment.com/> NASDAQ: ASGN People First. Find me on LinkedIn at: http://www.linkedin.com/pub/heidi-hawthorne/0/7b4/a39 ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 97, Issue 25 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- BEGIN-ANTISPAM-VOTING-LINKS ------------------------------------------------------ Teach SpamSniper if this mail (ID 01Gc7bWvy) is spam: Spam: http://admin.spamsniper.co.uk/canit/b.php?i=01Gc7bWvy&m=c326c3be8e02&t=20111222&c=s Not spam: http://admin.spamsniper.co.uk/canit/b.php?i=01Gc7bWvy&m=c326c3be8e02&t=20111222&c=n Forget vote: http://admin.spamsniper.co.uk/canit/b.php?i=01Gc7bWvy&m=c326c3be8e02&t=20111222&c=f ------------------------------------------------------ END-ANTISPAM-VOTING-LINKS
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