Andria, I purchased my HSV I/II from Cell Marque (RTU). They work very well with Dako Plus & Leica Bonds. These antibodies does NOT required antigen retrieval.
Dako Plus: 1) Deparafinize & hydration 2) Quench with Dako Dual Endogenous Block (5') 3) Incubate with 1st ab (HSV I or HSV II) (60') 4) Incubate with 2nd ab, Dako Envision+ M/R-HRP (30') 5) Envision+ DAB (5') 6) Mayer Hematoxylin (1') 7) TBST (30") 8) Dehydrate & Mount Leica Bonds (Refine DAB Kit); 1) Use Protocol "F" 2) Incubate 1st ab with NO AR "----"(Fill Open Vial with Cell Marque's HSV I/II) 3) DAB with DAB Enhancer Good Luck! Madeleine Supervisor Pathology - IPOX & Histology El Camino Hospital Mountain View, CA 94040 On Fri, Dec 23, 2011 at 10:00 AM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Happy Holidays (Brian-Prometheus) > 2. AUTO: Mills, Demaris is out of the office. (returning > 01/03/2012) (demaris.mi...@leica-microsystems.com) > 3. gram controls (Webb, Dorothy L) > 4. Antibody to Cryptococcus (Richard Cartun) > 5. HSV1 and HSV2 (Evans, Andria B) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 23 Dec 2011 10:42:39 -0500 > From: "Brian-Prometheus" <br...@prometheushealthcare.com> > Subject: [Histonet] Happy Holidays > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <007301ccc189$8081eae0$8185c0a0$@com> > Content-Type: text/plain; charset="us-ascii" > > > > Seasons Greetings from all of us here at Prometheus Healthcare! > > > > > > Brian Feldman > > Principal > > Prometheus Healthcare > > Office 301-693-9057 > > Fax 301-368-2478 > > > <http://us.mc538.mail.yahoo.com/mc/compose?to=br...@prometheushealthcare.com >> br...@prometheushealthcare.com > > <http://www.prometheushealthcare.com/> www.prometheushealthcare.com > > *** Stay up to date on the newest positions and healthcare trends nationwide > on Twitter!*** > > <http://twitter.com/PrometheusBlog> http://twitter.com/PrometheusBlog > > > > > > > > > > ------------------------------ > > Message: 2 > Date: Fri, 23 Dec 2011 10:01:29 -0600 > From: demaris.mi...@leica-microsystems.com > Subject: [Histonet] AUTO: Mills, Demaris is out of the office. > (returning 01/03/2012) > To: histonet@lists.utsouthwestern.edu > Message-ID: > > <ofbd4ab4d5.b83f58ab-on8625796f.00580714-8625796f.00580...@leica-microsystems.com> > > Content-Type: text/plain; charset=US-ASCII > > > I am out of the office until 01/03/2012. > > I will be out of the office on annual leave from Dec 21 through Jan 3. > > > Note: This is an automated response to your message "Histonet Digest, Vol > 97, Issue 27" sent on 12/23/2011 9:39:17 AM. > > This is the only notification you will receive while this person is away. > > > _____________________________________________________________________ > This e-mail has been scanned for viruses by Verizon Business Internet Managed > Scanning Services - powered by MessageLabs. For further information visit > http://www.verizonbusiness.com/uk > > > > ------------------------------ > > Message: 3 > Date: Fri, 23 Dec 2011 10:51:44 -0600 > From: "Webb, Dorothy L" <dorothy.l.w...@healthpartners.com> > Subject: [Histonet] gram controls > To: "'histonet@lists.utsouthwestern.edu'" > <histonet@lists.utsouthwestern.edu> > Message-ID: > > <65365f35c0f2ef4d846ec3ca73e49c43013480553...@hpemx3.healthpartners.int> > > Content-Type: text/plain; charset="us-ascii" > > I remember giving a couple of chicken livers to micro and they incubated them > overnight with gram +/-. I then cut those pieces smaller and processed them > in separate cassettes. This process has owrked real well for us. Was in the > Journal of Histotechnology but cannot remember which issue a few years back. > > Dorothy Webb > > > > ________________________________ > This e-mail and any files transmitted with it are confidential and are > intended solely for the use of the individual or entity to whom they are > addressed. If you are not the intended recipient or the individual > responsible for delivering the e-mail to the intended recipient, please be > advised that you have received this e-mail in error and that any use, > dissemination, forwarding, printing, or copying of this e-mail is strictly > prohibited. > > If you have received this e-mail in error, please immediately notify the > HealthPartners Support Center by telephone at (952) 967-6600. You will be > reimbursed for reasonable costs incurred in notifying us. HealthPartners > R001.0 > > > ------------------------------ > > Message: 4 > Date: Fri, 23 Dec 2011 12:34:51 -0500 > From: "Richard Cartun" <rcar...@harthosp.org> > Subject: [Histonet] Antibody to Cryptococcus > To: "Histonet" <Histonet@lists.utsouthwestern.edu> > Message-ID: <4ef4756b.7400.007...@harthosp.org> > Content-Type: text/plain; charset=US-ASCII > > Unfortunately, we have used the last micro-liter of our Cryptococcus antibody > that we obtained from DAKO years ago. Since DAKO no longer sells this > antibody I am wondering if anyone is using another commercially-available > antibody to label Cryptococcus in formalin-fixed tissue with > immunohistochemistry? > > Happy Holidays to all my friends and colleagues working in the field of > Histology. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > > > > > ------------------------------ > > Message: 5 > Date: Fri, 23 Dec 2011 12:48:37 -0500 > From: "Evans, Andria B" <aeva...@lghealth.org> > Subject: [Histonet] HSV1 and HSV2 > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <4182fdf23d7c9948bc41c4c082c3a54f043aac2fe...@mail-ag-cluster.lha.org> > Content-Type: text/plain; charset="iso-8859-1" > > I am currently having issues with our HSV1 and HSV2 staining tissue > components that it shouldn't be/background. We are running Ventana Benchmark > XTs and Ultras. Using Dako's polyclonal rabbit concentrate. Does anyone out > there in histoland have a concentration with a protocol that works and looks > clean? > > Thank you for your help! > > Andria B Evans HTL(ASCP)CM > This email was sent securely from the LGHealth Email Service > > Confidentiality Notice: > This e-mail message, including any attachments, is for the sole use > of intended recipient(s) and may contain confidential and > privileged information. > Any unauthorized review, use, disclosure or distribution is > prohibited. > If you are not the intended recipient, please contact the sender by > reply e-mail and destroy all copies of the original message. > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 97, Issue 28 > **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
