Do you do renal transplant? If yes, look for renal biopsies submitted for immunofluorescence studies, Let the Pathologists confirm that the biopsy is acute cellular rejection from renal transplant, because acute cellular rejection from kidney transplant is known to be positive for c4d. After the studies, cut some extra frozen sections from this biopsy and fix in acetone for 20 mins, and cover the slide with parafilm to avoid cracking of slide and store in the freezer. They are good for some months.
Tunde Ajibade BS, HTL(ASCP)QIHC Histology Supervisor Medical Center Hospital Odessa,TX Tel:432-640-2348 Fax:432-640-2303 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Martha Ward-Pathology Sent: Thursday, December 29, 2011 11:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] C4d immunofluorescent positive controls We are interested in finding out what type of positive control tissue everyone is using when performing C4d immunofluorescence on heart biopsies. Thanks in advance for any and all responses. Martha Ward, MT (ASCP) QIHC Manager, Molecular Diagnostics Lab Dept. of Pathology Wake Forest University Baptist Medical Center Winston-Salem, NC 27157 336-716-2104 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: The documents accompanying this email transmission contain confidential information belonging to the sender that is legally privileged. This information is intended only for the use of the individual or entity named above. The authorized recipient of this information is prohibited from disclosing this information to any other party and is required to destroy the information after its stated need has been fulfilled. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or action taken in reliance on the contents of these documents is strictly prohibited. If you have received this email in error, please notify the sender immediately to arrange for return of these documents. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet