Hi All,
I am setting up for a nerve fiber density test. The specifics of the procedure
I have been provided with are vague.
It basically involves taking a punch biopsy of skin, fixing in PLP,
cryoprotecting, embedding in a sucrose solution to a frozen stage, cover with
dry ice, and section using a sliding microtome at 50 microns. Then staining
the free-floating sections with a PGP 9.5 antibody.
If anyone is performing this testing, I would appreciate your response (you may
contact me privately if you wish).
Thanks!
Adrienne
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