If you are talking about a regressive H&E stain then perhaps it was 1% HCL acid in 70% alcohol
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sarah Dysart Sent: Thursday, January 05, 2012 11:00 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Amazing I find it amazing sometimes when you don't do something for awhile how quickly your brain throws the information away. That being said...I know back in the day when I was learning histology we used to make our own acid alcohol solution (now where I am had a butt load of Clearifier so I was using that up). I don't want to buy that stuff anymore as making the solution is way cheaper and works just as well. I want to say it was like a 1% acid solution in alcohol?? What was the acid? For some reason my brain says glacial acetic...but time has made me forget. Is the alcohol you mix it in 100% or something lower with a water content to it? Please help my alzheimers =) Happy Thursday!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet