Hi Peggy 1. I have found that to cut bone the block has to be REALLY cold. Face block with increased knife angle as suggested by Rene, and then place in freezer overnight - then try sectioning 2. Try surface decalcification of your block overnight in whichever soln. you normally use. 3. Section at 4-6 microns 4. You do not say, but high profile disposables work better with bone than low profile 5, failing this, you could try dewaxing and re decalcifying for a few more days
good luck On Tue, Jan 10, 2012 at 7:35 PM, DiCarlo, Margaret < mdica...@kaleidahealth.org> wrote: > Histonetters, > > > > I was given a cross section (3.5 x 3.2 x 0.6 cm) and a portion of a > femur that they think is osteoporotic. After decaling the cross section > of the femur in 10% formic acid for 12 days, processing using xylene and > embedding in Tissue Prep 2, I have been unsuccessful in attaining a > section. The knife just skips over the bone. I have soaked the bone > with a 50/50 solution of fabric softener and still can't get a section. > Does anyone have any suggestions? > > > > Thank you. > > > > Peggy DiCarlo HT (ASCP) > > Orthopedic Bone Lab > > Buffalo General Hospital > > Buffalo, NY 14203 > > 716-859-1293 > > > > > > The Keeping You Informed section of Kaleida Health’s website features a > wealth of information, stories and pictures about our valued workforce and > about the tremendous momentum our organization is experiencing. Check us > out at: www.kaleidahealth.org/kyi > > > CONFIDENTIALITY NOTICE: This email transmission and any documents, files, > or previous e-mail messages attached to it are confidential and intended > solely for the use of the individual or entity to whom they are addressed. > If you are not the intended recipient, or a person responsible for > delivering it to the intended recipient, you are hereby notified that any > further review, disclosure, copying, dissemination, distribution, or use of > any of the information contained in or attached to this e-mail transmission > is strictly prohibited. If you have received this message in error, please > notify the sender immediately by e-mail, discard any paper copies, and > delete all electronic files of the message. If you are unable to contact > the sender or you are not sure as to whether you are the intended > recipient, please e-mail ist...@kaleidahealth.org or call (716) 859-7777. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet