For all of you research guys out there working with this. I was asked to pose the question of how you quantify this stain. I know histology is a black and white yes/no kind of science, but I am being asked to quantify this stain. Are just the very positive cells counted as positive? There is what appears to be non-specific staining on some of the tumors (not all of them). This non-specific staining is only in the tumor and not in the surrounding normal tissue. Is this a sometimes artifact of the stain? For Ki-67 we are quantifying the stain using an average of positive cells per area. This gives you a sort of index to determine how Ki-67 positive each tumor is. The problem with the Caspase3 is that this fuzzy stain is in 2 of our tumors, and while I say read through it (non-specific staining) others are thinking it means something. Anyhoo, any help would be greatly appreciated. Basically how do you report your Caspase results? Just as a positive or negative (which every tumor will be positive) or what other method do you use. Thanks
Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
