Nancy Schmitt, Mouse anti-PAX 8 (Biocare, same dilution as spec. sheet);
1) Protocol F with Refine DAB kit 2) ER2 for 20 min Cell Pellet: 1) Pre-fix cell pellet with 10% formalin (~ 30min - 60min) @ RT 2) Spin down pre-fix cell in a 15 mi conical tube @ 4c, aspirate off supernatant (10% formalin) 3) Add pre-warm histogel (follow spec.sheet for warming gel) to cell pellet 4) Flick bottom of tube so cell pellet could mix with the pre-warm histogel 5) Chill tube immediately in ice-bucket to solidify the histogel (~ 10min - 20min) 6) Scrape gel mixture out with spatula & put on lens paper 7) Wrap lens paper & put in cassette for process If you need more more information, you can email me. Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) El Camino Hospital madelein...@elcaminohospital.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
