The MOM kits may minimize this but they do not eliminate the non specific binding completely in my experience, macrophages and plasma cells are particularly difficult to eliminate staining in.
Regards, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson Sent: Friday, January 06, 2012 9:13 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] A nit to pick - background IHC staining Happy Friday to you all! I just wanted to comment on the idea that when detecting mouse antibodies on mouse tissues gives you background staining. I consider background staining to be non-specific binding of some reagent to the tissue that is then detected with the chromogen or fluorophore. Anti-mouse antibodies specifically bind to the mouse Igs in the tissue as well as to the mouse Ig labeled antigen from the antibody. It's a nuisance and not specific to your target, but I don't consider it background. As previously mentioned, mouse on mouse kits work well to minimize this. Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet