I forgot to mention that I always use a D profile tungsten carbide permanent knife to make these sections.
Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: Patsy Ruegg [mailto:pru...@ihctech.net] Sent: Saturday, January 21, 2012 10:11 AM To: 'Douglas M Burns'; 'histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? I use the tape transfer system. What tissues are you trying to cut? If you are cutting soft tissues you should be using the 0.5 or 1x coated slides, the 4x and above are for bone. Have you tried putting your slides on a block of dryice after exposing them to uv, let them sit for a while to get really cold, then carefully pull the tape off while on the dryice I pull diagonally from corner to corner very slowly and smoothly. Cut the sections as thin as possible and do not press hard on the tape rolled onto the block but I do press hard and roll the heck of the tape on the coated slides, then expose to the uv 3 or 4 times before removing the tape. Patsy Ruegg, HT(ASCP)QIHC IHCtech 12635 Montview Blvd. Ste.215 Aurora, CO 80045 720-859-4060 fax 720-859-4110 www.ihctech.net www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas M Burns Sent: Wednesday, January 04, 2012 10:10 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Are there any CryoJane users out there who understand the quirks of the tape? Hello, Histonetters, To continue my (sad) string posts about CryoJane problems, we have now switched to the 4X slides, and we still observe the tape pulling pieces of the section off the slide. Sometimes the pieces are very small, sometimes they are large, and at times a whole region of the section comes off with the tape. We have now tried many different things to correct this: 1) pulling the tape off the section in many different ways, 2) pulling tape off at many different angles & speeds, 3) several different temperatures, 4) with many different mental attitudes, 5) with sections of different thickness, 6) light rolling of tape and section versus ferocious rolling, etc., etc. So far, no dice; we are puzzled. Does anyone have more ideas about what to adjust. We think that this really should work, but at the same time, we can't seem to locate many users of the CryoJane system. So, CryoJane enthusiasts, this is the time to talk about why you like it, or how you do it. thanks again --------------- Doug Burns, MBRF, Kansas City _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet