Re: CAP/ANP Policies Hello All,
I am desperate for helps! I just started my new job 6 months ago, and our lab has inspected by CAP. During their inspection, I received a big surprise because they found no written policies for Histology in our Q-Pulse. :( :( Obversely, we have a lot of deficiencies, mostly LACK of written policies, but no violations. :) I am swam with IHC/ISH QC & optimization on bench, and absolutely NO time for written policies (English is not my first language & strong skill). I would greatly appreciated if anyone would share their histology written policies with me (used as Template only & confidential). Following are our deficiencies that I need to submit to them within a month (probably need extension). General Quality Control ANP #s'; ANP.21050, 21100, 21150, 21350, 21366, 21382, 21390, 21395 Immunohistochemistry ANP #s'; ANP.22250, 22550, 22570, 22660, 22750, 22760, 22800, 22900, 22990, 22993 Equipment Maintenance ANP #s'; ANP.23048, 23050, 23075 Tissue Processor ANP #s'; ANP.23100, 23150 Paraffin Dispenser ANP #s'; ANP.23200, 23250, 23300 Floatation Baths ANP #s'; ANP.23350 Microtomes ANP #s'; ANP.23450 Automated Tissue Processor ANP #s'; ANP.24050 Mostly greatly any helps! Madeleine Huey BS, HTL (ASCP) QIHC Supervisor - Pathology (IPOX & Histology) madelein...@elcaminohospital.org On Sat, Jan 21, 2012 at 10:00 AM, <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. Galectin 3 and Tripsin (McMahon, Loralee A) > 2. Microtome Reicher-jung 2030 (Bustamante, Lin) > 3. K/L bone marrow (Lanigan, Christopher) > 4. Re: Tuberculosis positive tissue (Kim Donadio) > 5. Correct web address for FSH (Jerry Santiago) > 6. Re: RE: slide file storage to dry slides (Kim Donadio) > 7. Re: slide file storage to dry slides (Kim Donadio) > 8. sample woes (Patsy Ruegg) > 9. RE: A nit to pick - background IHC staining (Patsy Ruegg) > 10. RE: Are there any CryoJane users out there who understand the > quirks of the tape? (Patsy Ruegg) > 11. RE: Are there any CryoJane users out there who understand the > quirks of the tape? (Patsy Ruegg) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Fri, 20 Jan 2012 13:18:29 -0500 > From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu> > Subject: [Histonet] Galectin 3 and Tripsin > To: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: > > <ec3287d73321a14799bb96082de57b47265ef...@urmcms2.urmc-sh.rochester.edu> > > Content-Type: text/plain; charset="iso-8859-1" > > Hi Histonet, > > Wondering if anyone out there would be willing to share a company and > protocol for Galectin 3 and Trypsin IHC's on paraffin embedded tissue. > Thanks in advance. > > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > > > ------------------------------ > > Message: 2 > Date: Fri, 20 Jan 2012 19:14:34 +0000 > From: "Bustamante, Lin" <lbustama...@cvm.tamu.edu> > Subject: [Histonet] Microtome Reicher-jung 2030 > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <94b6dc15aaf2f046bf847d4c1ca9aac939c39...@cvmmb02.cvm.tamu.edu> > Content-Type: text/plain; charset="us-ascii" > > I am looking to buy 2 of this microtome in very good condition please. > Thank you. > Lin. > > Lin S. Bustamante, B.S., H.T.(ASCP) > VIBS Histology Lab Supervisor > College Of Veterinary Medicine > Texas A&M University > Phone (979) 845-3177 > Fax (979) 458-3499 > lbustama...@cvm.tamu.edu > > > > ------------------------------ > > Message: 3 > Date: Fri, 20 Jan 2012 14:17:23 -0500 > From: "Lanigan, Christopher" <lani...@ccf.org> > Subject: [Histonet] K/L bone marrow > To: histonet@lists.utsouthwestern.edu > Cc: cthorn...@dahlchase.com > Message-ID: > <9ddd0f026dc71b41b79d8e439d7951a30bab4...@cchsclexmb69.cc.ad.cchs.net> > Content-Type: text/plain; charset=us-ascii > > -----Original Message----- > > Date: Wed, 18 Jan 2012 08:29:48 -0500 > > From: Clare Thornton <cthorn...@dahlchase.com> > > Subject: [Histonet] K/L bone marrow > > To: "'histonet@lists.utsouthwestern.edu'" > > <histonet@lists.utsouthwestern.edu> > > Message-ID: > > <c9d78ffc9d668b4cbea4405f84697504f819b6f...@iris.dahlchase.net> > > Content-Type: text/plain; charset="us-ascii" > > > > Does anyone have a Kappa/Lambda bone marrow ISH protocol for use on the > Ventana Benchmark XT? > > > > thanks! > > Clare > > > > Clare J. Thornton, HTL(ASCP), QIHC > > Assistant Histology Supervisor > > Dahl-Chase Diagnostic Services > > 417 State Street, Suite 540 > > Bangor, ME 04401 > > cthorn...@dahlchase.com > > > > -----Reply----- > > > > Hi Clare, > > > > Sorry for the delay. > > > > Yes, I have a successful Kappa / Lambda ISH protocol for Bone Marrow on > the Ventana Benchmark XT. > > > > First, the software that you will need to have installed is called "XT > ISH Open Probes ChromogenicV3". This protocol will use the iView BLUE+ > DETECTION and with a RED STAIN II counterstain. > > > > Before I get to the protocol, I'll fill you in on the Kappa and Lambda > probes. They do NOT arrive in a dispenser. They each arrive in 4 > pre-diluted vials, so you will need to fill a "user-fillable dispenser". > All four pre-diluted vials are mixed together into one user-fillable > dispenser. Apparently, Ventana is required to package each separately. > > > > Additionally, you will need to run a control. This control will confirm > the presence of non-degradated RNA. The control I used was U6, and the > protocol is identical to the Kappa or Lambda except for the selected ISH > probe. > > > > By the way, the selected ISH probe will likely be "ISH PROBE 1" or "ISH > PROBE 2" because the user-fillable dispenser will not have the proper > commercial bar code. > > > > One last thing, I was concerned initially about Bone Marrow adhesion to > the charged slides, so I ran a comparison of 2 leading brands. The > clear winner was Superfrost Plus distributed by Cardinal Health. > > > > Finally, the following is a successful protocol summary. > > > > 1 Baking [Selected] > > 2 Warmup Slide to [69 Deg C], and Incubate for [20 Minutes] ( Baking ) > > 3 Deparaffinization [Selected] > > 4 Standard [Selected] > > 5 Warmup Slide to [69 Deg C], and Incubate for 4 Minutes ( > Deparaffinization ) > > 6 Enzyme [Selected] > > 7 Apply Coverslip, One Drop of [ISH-PROTEASE 2] ( Enzyme ), and Incubate > for [8 Minutes] > > 8 Probe [Selected] > > 9 Probe Auto Dispense [Selected] > > 10 1 Drop of Probe Dispensed [Selected] > > 11 Apply One Drop of [ISH Probe 1] ( ISH Probe ), Apply Coverslip, and > Incubate for 4 Minutes > > 12 Denature [Selected] > > 13 Warmup Slide to [75 Deg C], and Incubate for [4 Minutes] ( > Denaturation ) > > 14 Hybridization [Selected] > > 15 Warmup Slide to [44 Deg C], and Incubate for 4 Minutes ( > Hybridization ) > > 16 Incubate for [1 Hour] ( Hybridization ) > > 17 Stringency Washes [Selected] > > 18 Stringency Wash #1 [Selected] > > 19 Warmup Slide to [60 Deg C], and Incubate for [8 Minutes] ( Stringency > Wash #1 ) > > 20 Stringency Wash #2 [Selected] > > 21 Incubate for [8 Minutes] ( Stringency Wash #2 ) > > 22 Stringency Wash #3 [Selected] > > 23 Incubate for [8 Minutes] ( Stringency Wash #3 ) > > 24 Detection Kit [Selected] > > 25 Blue Detection [Selected] > > 26 Incubate for [32 Minutes] ( Substrate ) > > 27 Counterstain [Selected] > > 28 Apply One Drop of [Red Stain II] ( Counterstain ), Apply Coverslip, > and Incubate for [4 Minutes] > > 29 Post Run LCS Application [Selected] > > > > Christopher Lanigan > > Research Technologist > > Molecular Pathology > > Cleveland Clinic Foundation > > 9500 Euclid Avenue L3-127 > > Cleveland, OH 44195 > > > > > > > > > =================================== > > Please consider the environment before printing this e-mail > > Cleveland Clinic is ranked one of the top hospitals > in America by U.S.News & World Report (2010). > Visit us online at http://www.clevelandclinic.org for > a complete listing of our services, staff and > locations. > > > Confidentiality Note: This message is intended for use > only by the individual or entity to which it is addressed > and may contain information that is privileged, > confidential, and exempt from disclosure under applicable > law. If the reader of this message is not the intended > recipient or the employee or agent responsible for > delivering the message to the intended recipient, you are > hereby notified that any dissemination, distribution or > copying of this communication is strictly prohibited. If > you have received this communication in error, please > contact the sender immediately and destroy the material in > its entirety, whether electronic or hard copy. Thank you. > > > ------------------------------ > > Message: 4 > Date: Fri, 20 Jan 2012 17:25:52 -0500 > From: Kim Donadio <one_angel_sec...@yahoo.com> > Subject: Re: [Histonet] Tuberculosis positive tissue > To: Kim Donadio <one_angel_sec...@yahoo.com> > Cc: "Histonet@lists.utsouthwestern.edu" > <Histonet@lists.utsouthwestern.edu> > Message-ID: <8876deb4-0c35-4867-93ba-46a4fde6c...@yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Oops. 10% bleach to clean. They do have some stuff called tuberculicide < > spelled wrong I'm sure. Oh and get a tine test to see if you had exposure. > Sorry for my half reply earlier. Was on break at work with ten things in > head. Hope you havnt had an exposure > Best wishes > Kim Donadio > > Sent from my iPhone > > On Jan 20, 2012, at 10:06 AM, Kim Donadio <one_angel_sec...@yahoo.com> wrote: > >> If your in a hospital: I've always had it verified with the microbiology >> department if it hasn't been done already(if not then still the following) >> Then it needs to be reported to your local department of health and the CDC >> since it is a reportable disease. I'm sure others can expand on this but >> this is what I've had to do. >> Kim Donadio >> >> Sent from my iPhone >> >> On Jan 20, 2012, at 6:47 AM, Michele Email <michelecar...@yahoo.com> wrote: >> >>> Hi everyone, was wondering what procedure you have when you find out after >>> the fact that the tissue is positive for TB. What Decontamination >>> procedures do you perform? Also what about documentation? Any help would >>> be appreciated. >>> Thank you >>> Michele Carr >>> >>> >>> Sent from my iPad >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 5 > Date: Fri, 20 Jan 2012 16:21:50 -0800 (PST) > From: Jerry Santiago <jerry.santi...@bellsouth.net> > Subject: [Histonet] Correct web address for FSH > To: histonet@lists.utsouthwestern.edu > Message-ID: > <1327105310.35635.yahoomai...@web181711.mail.ne1.yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Sorry guys, > > I posted the wrong link o the Florida Society for Histotechnology website. The > web address is www.fshgroup.org. > > > ------------------------------ > > Message: 6 > Date: Fri, 20 Jan 2012 21:43:21 -0500 > From: Kim Donadio <one_angel_sec...@yahoo.com> > Subject: Re: [Histonet] RE: slide file storage to dry slides > To: "dkb...@chs.net" <dkb...@chs.net> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu>, "Weems, Joyce" > <jwe...@sjha.org> > Message-ID: <35472ae1-2384-4b12-b444-3145805cd...@yahoo.com> > Content-Type: text/plain; charset=us-ascii > > Yeah. I want this median too. Thanks for heads up > Kim > > Sent from my iPhone > > On Jan 19, 2012, at 10:25 AM, dkb...@chs.net wrote: > >> Joyce, >> Interesting! What methodology are using to remove the coverslip and with >> what difficulty? I may be interested in changing to this medium. Are you >> using this same medium with Non-gyn Cytology and have you had any bleeding >> problems? Also we do not use Xylene. We use a substitute. >> Thanks! >> Debbie >> >> Debbie M. Boyd, HT(ASCP) l Chief Histologist l Southside Regional Medical >> Center I >> 200 Medical Park Boulevard l Petersburg, Va. 23805 l T: 804-765-5050 l F: >> 804-765-5582 l dkb...@chs.net >> >> >> >> >> >> >> >> "Weems, Joyce" <jwe...@sjha.org> >> Sent by: histonet-boun...@lists.utsouthwestern.edu >> 01/19/2012 09:58 AM >> >> To >> Sebree Linda A <lseb...@uwhealth.org>, "Histonet@lists.utsouthwestern.edu" >> <Histonet@lists.utsouthwestern.edu> >> cc >> >> Subject >> [Histonet] RE: slide file storage to dry slides >> >> >> >> >> >> >> We use fast dry mounting media from ThermoFisher Scientific - Item# 22 050 >> 102 - that doesn't need extra drying. File the next day with no sticking.. >> j >> >> >> Joyce Weems >> Pathology Manager >> Saint Joseph's Hospital >> 5665 Peachtree Dunwoody Rd NE >> Atlanta, GA 30342 >> 678-843-7376 - Phone >> 678-843-7831 - Fax >> >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sebree >> Linda A >> Sent: Thursday, January 19, 2012 09:26 >> To: Histonet@lists.utsouthwestern.edu >> Subject: [Histonet] slide file storage to dry slides >> >> Good morning all, >> >> We've recently switched from film coverslipping back to glass and >> therefore need to thoroughly dry our slides before permanent filing. I >> recall, in my first histology job....30 + years ago, that we used metal >> stacking slide files that you could put an insert into the drawers that >> looked like a non-stretchy spring. The wires of this "spring" held the >> slides apart to dry, then they could be filed without the "spring" when >> they were completely dry. >> >> Anyone know if that product still exists? Or does anyone have a better >> solution for drying slides while still keeping them in order? >> >> Thanks for the assist, >> >> Linda >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> Confidentiality Notice: >> This e-mail, including any attachments is the >> property of Catholic Health East and is intended >> for the sole use of the intended recipient(s). >> It may contain information that is privileged and >> confidential. Any unauthorized review, use, >> disclosure, or distribution is prohibited. If you are >> not the intended recipient, please delete this message, and >> reply to the sender regarding the error in a separate email. >> >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> >> >> -------------------------------------------------------------------------- >> Disclaimer: This electronic message may contain information that is >> Proprietary, Confidential, or legally privileged or protected. It >> is intended only for the use of the individual(s) and entity named >> in the message. If you are not an intended recipient of this >> message, please notify the sender immediately and delete the >> material from your computer. Do not deliver, distribute or copy >> this message and do not disclose its contents or take any action in >> reliance on the information it contains. >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 7 > Date: Fri, 20 Jan 2012 21:50:43 -0500 > From: Kim Donadio <one_angel_sec...@yahoo.com> > Subject: Re: [Histonet] slide file storage to dry slides > To: Sebree Linda A <lseb...@uwhealth.org> > Cc: "<Histonet@lists.utsouthwestern.edu>" > <Histonet@lists.utsouthwestern.edu> > Message-ID: <64b3fbd5-2057-4108-b96e-bef9ebcc6...@yahoo.com> > Content-Type: text/plain; charset=us-ascii > > I've seen this a couple ways. Metal trays put in slide drying oven on low > temp overnight. Low or you will ruin some labels . I've also seen these nice > wooden boxes that hold the metal slide trays. You put them in it , it's like > a rack . Put them in order. I think they hold about 1000 slides. You just > leave these there they will be your most recent. By the time it's full. Put > half up and then continue rotating after that. I'd check with fisher maybe > Nite nite > Kim > > Sent from my iPhone > > On Jan 19, 2012, at 9:25 AM, "Sebree Linda A" <lseb...@uwhealth.org> wrote: > >> Good morning all, >> >> We've recently switched from film coverslipping back to glass and >> therefore need to thoroughly dry our slides before permanent filing. I >> recall, in my first histology job....30 + years ago, that we used metal >> stacking slide files that you could put an insert into the drawers that >> looked like a non-stretchy spring. The wires of this "spring" held the >> slides apart to dry, then they could be filed without the "spring" when >> they were completely dry. >> >> Anyone know if that product still exists? Or does anyone have a better >> solution for drying slides while still keeping them in order? >> >> Thanks for the assist, >> >> Linda >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Sat, 21 Jan 2012 09:39:03 -0700 > From: "Patsy Ruegg" <pru...@ihctech.net> > Subject: [Histonet] sample woes > To: "'Histonet'" <histonet@lists.utsouthwestern.edu> > Message-ID: <43722E05B4DA45048652C318FB96543D@prueggihctechlt> > Content-Type: text/plain; charset="iso-8859-7" > > Please advise. > > > > I was sent a bunch of sample by an investigator who is not too sharp. The > samples were prepared thus: > > > > Pancreases were removed, washed placed in a modified Zamboni fixative (2% > formaldehyde, 15%Picric acid in 0.1M PBS, pH 7.5). Sample portions were cut > from the head, body and tail of the pancreas in 3 mm by 3 mm sections. > > The pancreatic tissue samples from the various regions of the pancreas were > fixed overnight; samples were equilibrated in 50% sucrose in 0.01M PBS for > 12 hours at 4°C, and mounted in Tissue Tek OCT Compound (Miles Inc.); 0.015 > mm (15 ìm) thick sections were obtained by cryostat sectioning. > > SAMPLES WERE FROZEN IN 50% Tissue Tek 50% sucrose , then shipped on wet ice, > not dry ice. > > These frozen tissue samples were in small embedding molds with a tiny dab of > the OCT/sucrose gel on top of them, it looked like none was under them. They > shipped these supposedly previous frozen samples to me on wet ice, not dry > ice (dumb move), the molds were open on top in a box, the sample thawed and > the gel and sample was leaking out of their molds, it was a mess. The > investigator who sent the samples to me said this "the samples arrived > within 18hrs as signed for, so they should have still been frozen", how this > person got to be the CEO of a Biotech company I cannot imagine. > > > > They wanted me to just refreeze them and cryosection them to test some abs > they sent with them, I said that I would not do that, I did agree to take 2 > samples as a pilot study and fix them in formalin overnight and then process > and embed them in paraffin, which I have done. If I can get them to section > (they are still soft and squishy ) I was planning on doing an H&E stain and > look at the samples to see if they look preserved at all. My question to you > all is this: if the tissue looks viable by H&E what should I try to test > immuno reactivity viability? I was thinking of running an antibody like > vimentin or cytokeratin or something, but these are ms pancreas so maybe I > should use insulin ab or something else. > > > > Thank you for your valuable advise, > > > > Regards, > > Patsy > > > > > > Patsy Ruegg, HT(ASCP)QIHC > > IHCtech > > 12635 Montview Blvd. Ste.215 > > Aurora, CO 80045 > > 720-859-4060 > > fax 720-859-4110 > > www.ihctech.net > > www.ihcrg.org > > > > > > ------------------------------ > > Message: 9 > Date: Sat, 21 Jan 2012 10:04:58 -0700 > From: "Patsy Ruegg" <pru...@ihctech.net> > Subject: RE: [Histonet] A nit to pick - background IHC staining > To: "'Theresa \(Teri\) Johnson'" <tjohn...@gnf.org>, > <histonet@lists.utsouthwestern.edu> > Message-ID: <94A5152BB90D45D2A421C0173304B708@prueggihctechlt> > Content-Type: text/plain; charset="us-ascii" > > The MOM kits may minimize this but they do not eliminate the non specific > binding completely in my experience, macrophages and plasma cells are > particularly difficult to eliminate staining in. > > Regards, > Patsy > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Theresa > (Teri) Johnson > Sent: Friday, January 06, 2012 9:13 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] A nit to pick - background IHC staining > > Happy Friday to you all! > > I just wanted to comment on the idea that when detecting mouse antibodies on > mouse tissues gives you background staining. I consider background staining > to be non-specific binding of some reagent to the tissue that is then > detected with the chromogen or fluorophore. Anti-mouse antibodies > specifically bind to the mouse Igs in the tissue as well as to the mouse Ig > labeled antigen from the antibody. > > It's a nuisance and not specific to your target, but I don't consider it > background. As previously mentioned, mouse on mouse kits work well to > minimize this. > > Teri Johnson, HT(ASCP)QIHC > GNF Histology Lab Manager > Genomics Institute of the Novartis Research Foundation > 858-332-4752 > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 10 > Date: Sat, 21 Jan 2012 10:11:29 -0700 > From: "Patsy Ruegg" <pru...@ihctech.net> > Subject: RE: [Histonet] Are there any CryoJane users out there who > understand the quirks of the tape? > To: "'Douglas M Burns'" <dmbur...@gmail.com>, > <histonet@lists.utsouthwestern.edu> > Message-ID: <9B4BBD01D2E94FD3BE86E17A799414CC@prueggihctechlt> > Content-Type: text/plain; charset="us-ascii" > > I use the tape transfer system. What tissues are you trying to cut? If you > are cutting soft tissues you should be using the 0.5 or 1x coated slides, > the 4x and above are for bone. > > Have you tried putting your slides on a block of dryice after exposing them > to uv, let them sit for a while to get really cold, then carefully pull the > tape off while on the dryice I pull diagonally from corner to corner very > slowly and smoothly. Cut the sections as thin as possible and do not press > hard on the tape rolled onto the block but I do press hard and roll the heck > of the tape on the coated slides, then expose to the uv 3 or 4 times before > removing the tape. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas M > Burns > Sent: Wednesday, January 04, 2012 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Are there any CryoJane users out there who understand > the quirks of the tape? > > Hello, Histonetters, > > To continue my (sad) string posts about CryoJane problems, we have now > switched to the 4X slides, and we still observe the tape pulling pieces of > the section off the slide. Sometimes the pieces are very small, sometimes > they are large, and at times a whole region of the section comes off with > the tape. > > We have now tried many different things to correct this: 1) pulling > the tape off the section in many different ways, 2) pulling tape off at > many different angles & speeds, 3) several different temperatures, 4) with > many different mental attitudes, 5) with sections of different thickness, > 6) light rolling of tape and section versus ferocious rolling, etc., etc. > So far, no dice; we are puzzled. > > Does anyone have more ideas about what to adjust. We think that this > really should work, but at the same time, we can't seem to locate many > users of the CryoJane system. So, CryoJane enthusiasts, this is the time to > talk about why you like it, or how you do it. > > thanks again --------------- Doug Burns, MBRF, > Kansas City > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > Message: 11 > Date: Sat, 21 Jan 2012 10:13:52 -0700 > From: "Patsy Ruegg" <pru...@ihctech.net> > Subject: RE: [Histonet] Are there any CryoJane users out there who > understand the quirks of the tape? > To: "'Patsy Ruegg'" <pru...@ihctech.net>, "'Douglas M Burns'" > <dmbur...@gmail.com>, <histonet@lists.utsouthwestern.edu> > Message-ID: <0A13102785964D6F8F4AF4DCAE56A872@prueggihctechlt> > Content-Type: text/plain; charset="us-ascii" > > I forgot to mention that I always use a D profile tungsten carbide permanent > knife to make these sections. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: Patsy Ruegg [mailto:pru...@ihctech.net] > Sent: Saturday, January 21, 2012 10:11 AM > To: 'Douglas M Burns'; 'histonet@lists.utsouthwestern.edu' > Subject: RE: [Histonet] Are there any CryoJane users out there who > understand the quirks of the tape? > > I use the tape transfer system. What tissues are you trying to cut? If you > are cutting soft tissues you should be using the 0.5 or 1x coated slides, > the 4x and above are for bone. > > Have you tried putting your slides on a block of dryice after exposing them > to uv, let them sit for a while to get really cold, then carefully pull the > tape off while on the dryice I pull diagonally from corner to corner very > slowly and smoothly. Cut the sections as thin as possible and do not press > hard on the tape rolled onto the block but I do press hard and roll the heck > of the tape on the coated slides, then expose to the uv 3 or 4 times before > removing the tape. > > Patsy Ruegg, HT(ASCP)QIHC > IHCtech > 12635 Montview Blvd. Ste.215 > Aurora, CO 80045 > 720-859-4060 > fax 720-859-4110 > www.ihctech.net > www.ihcrg.org > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Douglas M > Burns > Sent: Wednesday, January 04, 2012 10:10 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Are there any CryoJane users out there who understand > the quirks of the tape? > > Hello, Histonetters, > > To continue my (sad) string posts about CryoJane problems, we have now > switched to the 4X slides, and we still observe the tape pulling pieces of > the section off the slide. Sometimes the pieces are very small, sometimes > they are large, and at times a whole region of the section comes off with > the tape. > > We have now tried many different things to correct this: 1) pulling > the tape off the section in many different ways, 2) pulling tape off at > many different angles & speeds, 3) several different temperatures, 4) with > many different mental attitudes, 5) with sections of different thickness, > 6) light rolling of tape and section versus ferocious rolling, etc., etc. > So far, no dice; we are puzzled. > > Does anyone have more ideas about what to adjust. We think that this > really should work, but at the same time, we can't seem to locate many > users of the CryoJane system. So, CryoJane enthusiasts, this is the time to > talk about why you like it, or how you do it. > > thanks again --------------- Doug Burns, MBRF, > Kansas City > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 98, Issue 28 > **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
