There is no "rapid solution" to fixing fatty tissue. By its own nature of being "fatty" it hampers the formalin penetration and require more time than other tissues. If you use alcoholic-formalin, you are fixing it with ethanol and with formalin INDEPENDENTLY because each will penetrate the tissue at a different rate. If you cut the fixation time you will end with an alcoholic fixation, reinforced with the dehydrating alcohols, but it will be lacking the formalin fixation. Take your time and you will avoid improper infiltration consequence of an improper fixation. René J.
--- On Mon, 2/6/12, Dawn Oakes <[email protected]> wrote: From: Dawn Oakes <[email protected]> Subject: [Histonet] FATTY TISSUSE To: "[email protected]" <[email protected]> Date: Monday, February 6, 2012, 1:17 PM We would like to know what rapid fix solution is being used prior to putting fatty tissues on the tissue processor with alcoholic formalin.? We do not have the availability of a separate program. Thanks , Dawn Oakes HT ASCP Olympic Medical Center 939 Caroline Street Port Angeles, WA 98362 360-417-7467 ------------------------------------------------------------------------- Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended individual(s) named above and may contain confidential, privileged, and/or protected information. Any unauthorized review, use, disclosure, copying, or distribution of its contents is prohibited. If you are not the intended recipient, you have received this email in error. If so, please notify the sender immediately by reply email and delete/destroy the original and all copies of this communication. Also know that Internet e-mail is not secure. In choosing to communicate with Olympic Medical Center by email you will assume these confidentiality risks. Internet messages may become corrupted, incomplete, or may incorrectly identify the sender. _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
