I think these granules are of insoluble hematoxylin or preceptated hematoxylin granules a had these condition 2 years ago and resolved it by prepairing new one. hope these could help  Mohamed Abd el Razik Ass. Lec. of veterinary histology Cairo University- Egypt
________________________________ From: Rolly Perez <rpe...@medsurgpath.com> To: HISTONET <histonet@lists.utsouthwestern.edu> Sent: Thursday, February 9, 2012 11:34 PM Subject: [Histonet] Re-send Darker &/or granular hematoxylin staining (no photo) Hello my colleagues, Any input will be much appreciated - what can cause darker and/or granular hematoxylin staining on the goblet cells (mucin areas) of certain GI biopsies while on other similar GIs in the very same rack of slides, they stain water clear which is the preferred outcome. I use regressive H&E staining using Sigma Aldrich Harris hemtoxylin & standard 1% Acid alcohol (hydrocholric acid). I tried stronger acid for differentiation, cut thinner, longer time in acid differentiation, less hematoxylin time, to try to get the mucin staining as minimal as possible but I can't seem to eliminate the darker &/or more granular staining on some GIs. Thanks in advance for any thoughts you might have! Rolly Perez, HT(ASCP) Histology Supervisor MedSurg Pathology Associates, Inc. 503.443.2157 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet