Recommended by who?
Mike

-----Original Message-----
From: [email protected] 
[mailto:[email protected]] On Behalf Of Jennifer 
MacDonald
Sent: Friday, February 10, 2012 2:43 PM
To: Rene J Buesa
Cc: [email protected]; Stephanie Hoyle-Thacker; 
[email protected]
Subject: Re: [Histonet] AFB control


It is recommended to run a negative AFB control to rule out false 
positives.  Some sources of water have acid fast organisms, not TB, and if 
the water is used in the water baths or for staining you could pick up 
those organisms.  The recommendation is to cut a section of tissue, known 
NOT to contain AFB organisms, and run it through with the positive control 
and patient.  You would pick it up on the same water bath that you used 
for the patient.




Rene J Buesa <[email protected]> 
Sent by: [email protected]
02/10/2012 11:55 AM

To
[email protected], Stephanie Hoyle-Thacker 
<[email protected]>
cc

Subject
Re: [Histonet] AFB control






All I recall about controls in HC procedures (any of them) is to have a 
(+) control. The only one that requires both (-) and (+) is PAS René J.

--- On Fri, 2/10/12, Stephanie Hoyle-Thacker <[email protected]> 
wrote:


From: Stephanie Hoyle-Thacker <[email protected]>
Subject: [Histonet] AFB control
To: [email protected]
Date: Friday, February 10, 2012, 1:52 PM



Is anyone using a negative control for AFB stains?  If so is there a 
regulation that states negative controls should be run for AFB stains.  I 
seem to recall seeing such a regulation but cannot find it.

Thanks for your help!

Renee Thacker                           
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