I don't know about implants but we were having a terrible time with bone marrows coming off. Usually they came off during the HEIR using the steamer. At that point we were placing our slides lying flat in a 55-60º oven overnight which usually helps tremendously with difficult tissue. This was followed with routine deparaffinization and hydration. It was suggested to us to try a 70º water bath for 2-4 hours for HEIR. I was doubtful that my antibodies would work but they did. In fact the staining was better with 2 hr HEIR than with 4.
Donna Reynolds Chief Histology laboratory, Research Department Cancer Biology U.T. M.D. Anderson Cancer Center, Houston, TX. 713-792-8106 Message: 4 Date: Mon, 13 Feb 2012 09:53:15 -0600 From: "del phillips" <dphill...@vetmed.lsu.edu> Subject: [Histonet] IHC and bone implants To: <Histonet@lists.utsouthwestern.edu> Message-ID: <000a01ccea67$9958da90$cc0a8fb0$@vetmed.lsu.edu> Content-Type: text/plain; charset="us-ascii" Does anyone have any suggestions for staining bone implants? I am having trouble with wash offs. I use charged slides and they are still washing off. Thanks Del _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
