Hello This is my first post to this site. I have encountered a problem with embedding of mice heads in Paraffin (Gurr). Initially with Embryonic day 13-14, the protocol was working properly and the sections were also nice but with Embryonic day 17-19 I could see even after several hours of ethanol wash and xylene treatment the mice heads were soft but still I embedded the samples in paraffin. When I removed the paraffin blocks from the mold I could see the mice portion to be white, some flecks of wax coming out of surface and the surface of wax had some cracks. I could not rule out what was causing the problem.
Protocol I used for E 17-19 After Fixing in EDTA (2 weeks) and putting the mice heads in running water for 48 hours, 50% Ethanol 3 hours 70% Ethanol 3 hours 95% Ethanol 3 hours 100% Ethanol 3 hours 100% Ethanol 3 hours Xylene 2-4 hrs (till clearing of samples was observed) Paraffin 30 mins Paraffin 30 mins Paraffin 30 mins Paraffin 30 mins Could anybody share me your protocol for Embryonic day 17-19 and Post natal days. Regards Mote _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
