We are not using negative controls. We only add positive controls at the bottom 
of our patient slides. Sometimes there are 3 different types of control tissue 
(a TMA), so that you can use the same control block for several antibodies. You 
could add a negative control in the same block as the positive control and add 
these at the bottom of your slides (or at the top, if you wish).
 
 
Willem Hoekert
OLVG
The Netherlands

________________________________

Van: histonet-boun...@lists.utsouthwestern.edu namens Tom McNemar
Verzonden: do 16-2-2012 16:27
Aan: histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet] IHC and negative controls?



Hi All,

We have one Ventana XT and often exceed the slide capacity.  Our pathologists 
no longer want us to run negatives and will use internal negatives instead.  Is 
this common practice?  Thanks.  I appreciate any feedback.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcne...@lmhealth.org<mailto:tmcne...@lmhealth.org>
www.LMHealth.org<file:///C:\Documents%20and%20Settings\TMCNEMAR\Application%20Data\Microsoft\Signatures\www.LMHealth.org
 
<file:///C:/Documents%20and%20Settings/TMCNEMAR/Application%20Data/Microsoft/Signatures/www.LMHealth.org>
 >

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