Hi Dorothy If you want to prolong the viability of pre-cut unstained slides, firstly dry them, then dunk the slide into a bath of molten paraffin wax deep enough to submerge all of the tissue section, then remove it and allow the wax coating to solidify. This will keep the environment out of your cut section. When you need to use the slides, simply heat to melt the excess wax and then dewax and rehydrate in the usual manner.
Regards, Mark Bromley. Manager, Histopathology. ___________________________________________ Melbourne Pathology. 103 Victoria Pde, Melbourne. | (03) 92877806 www.mps.com.au -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Webb, Dorothy L Sent: Thursday, 23 February 2012 5:18 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] questions For those of you who use reagent alcohol, have you ever experienced any problems in processing or staining, such as artifacts, crystals forming, etc?? How long are unstained slides usable? Do any of you pick up extra sections from a ribbon if the tissue is minimal ? We do and have used them at times, but a pathologist would like them saved "forever" in case tissue is needed for a molecular test and there is not enough left in the block. Appreciate ahead of time your responses! Dorothy Webb, HT (ASCP) Regions Hospital ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet