We have run into an interesting scenario and wondering what the "experts" think! We cut bone marrow bx's and lymph nodes for lymphoma @ 3 microns on one particular microtome. Within the past month, the hematopathologist has felt the sections are thicker than the usual 3 microns. I had our service technician measure the microns and the equipment was cutting as set. I had the blocks cut on a different microtome and we have seen variations there also. My question is, does the amount of time on ice make a minor difference in the section thickness? I know a lot of responses may be the difference in the tech cutting inasmuch as how fast they turn the rotations, etc., but,we have ruled out that variable by having more than one tech cut at the microtome in question. I am stymied as to how to remedy this fluctuation! This is why we love histology, so many variables to create a problem and why I love histonet, so many techs to help one through a dilemma!! Thank you!!
Dorothy Webb, HT (ASCP) ________________________________ This e-mail and any files transmitted with it are confidential and are intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient or the individual responsible for delivering the e-mail to the intended recipient, please be advised that you have received this e-mail in error and that any use, dissemination, forwarding, printing, or copying of this e-mail is strictly prohibited. If you have received this communication in error, please return it to the sender immediately and delete the original message and any copy of it from your computer system. If you have any questions concerning this message, please contact the sender. Disclaimer R001.0 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
