As far as i know, osteoid will not show up on a decalcified section. On Thu, Mar 8, 2012 at 6:00 PM, Grantham, Andrea L - (algranth) < algra...@email.arizona.edu> wrote:
> Hopefully a hard tissue guru is out there and can help with this. > > A PI here is asking about doing a Goldner's stain on mouse femur. I'm not > set up to cut sections of undecalcified bone so my questions are: > 1. can this stain be done successfully on decalcified bone? > 2. if so, what type of decal should be used - EDTA? > 3. I have googled the stain but didn't see what the preferred thickness > was for this stain. > > Need this info fairly soon! The bones are on the way. > > Thanks!!! > > Andi G. > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa +27 11 717 2298 (tel & fax) 073 5574456 (emergencies only) Question: Are rhinos overweight unicorns? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet