Stacy, You can buy a complete Brown and Brenn staining kit from Newcomer Supply which is very good, and not change your method. The picric acid/acetone mixture is included and you wouldn't have to store stock picric acid nor ether in the lab but just this small amount of Picric acid/acetone that comes in the kit. Disposal should not be that much of a problem. They also have a Hucker Twort Gram stain that uses acetone for destaining and you supply the acetone. Poly Scientific has Gram Stain kit (Hucker modification) which uses Grams decolorizer, a mixture of acetone and alcohol. They have excellent staining kits too.
Gayle Callis HTL/HT/MT(ASCP) -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Giroux, Stacy Sent: Monday, March 12, 2012 11:36 AM To: [email protected] Subject: [Histonet] Gram Stain Hi, Our lab is currently transitioning from the Brown & Brenn gram stain due to no longer wanting to store picric acid due to its potential hazards. Our pathologists have requested a gram stain for paraffin embedded tissue that looks similar but does not use picric acid or ether. Does anyone have any suggestions on stains that could be used or gram stain kits that are available for purchase that are good? Thank you for your help, Stacy CONFIDENTIALITY NOTICE: This email message and any accompanying data or files is confidential and may contain privileged information intended only for the named recipient(s). If you are not the intended recipient(s), you are hereby notified that the dissemination, distribution, and or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at the email address above, delete this email from your computer, and destroy any copies in any form immediately. Receipt by anyone other than the named recipient(s) is not a waiver of any attorney-client, work product, or other applicable privilege._______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
