No, If you need to demonstrate that PAS positive material is glycogen and not some other carbohydrate containing material you must do a PAS on the test case as well.
(see my earlier Post) Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Theresa (Teri) Johnson Sent: Friday, 16 March 2012 3:22 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: PAS Diastase I disagree that two patient slides are needed. What is needed for diagnosis is one patient slide with digestion, and two control slides, one with and one without. If the control slide shows adequate digestion of the glycogen and the other control slide shows adequate PAS positivity, the pathologist can be assured that if the patient slide has any PAS positivity it is not due to glycogen. I am guessing this is only in cases where they are not concerned with glycogen storage as part of the diagnosis (liver biopsy). Ask your pathologist what he or she is looking for. My guess is it is not glycogen, but some other PAS positive component. On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: > Hi All, > At a local lab when a pathologist orders a PAS diastase the > histotechnicians do just one slide with diastase. They do not do an > undigested slide. How would the pathologist know if the digested > slide had a glycogen to begin with? Am I over thinking this? > Thanks, > Jennifer > Teri Johnson, HT(ASCP)QIHC GNF Histology Lab Manager Genomics Institute of the Novartis Research Foundation 858-332-4752 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet