1. Try an alcoholic fixative like formalin-alcohol-acetic acid
2. Try putting Safranin O in the fixative. Shepard and Mitchell, J. Ultrastruc. Res. 54:451-460, 1976. or same authors used Toluidine Blue O J. Histochem. Cytochem. 24:621-629, 1976. Geoff On 3/15/2012 8:41 PM, Jennifer Scholler wrote:
Hello, I am attempting to stain fetal membranes to look at the GAG content. My advisor suggested Safronin O, which I tried on a preliminary set of slides (hematoxylin, fast green, then safronin O). I did get some staining and saw a band of red staining between the layers, as expected, but the staining is not nearly as pronounced as in images of Safronin O stained tissues that I have seen (some of this obviously may be due to imaging settings). Also, after doing some reading, it seems that Alcian blue may be another option. Can anyone with experience staining FMs or similar tissues suggest a stain and protocol for this? Thanks! Regards, Jennifer Scholler M.S. candidate Mechanical Engineering University of Colorado at Boulder _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
-- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
