I think normal breast staining of myoepithial cells is the best control for this antibody. We use BioGenex's antibody (product # MU333-UC) at 1:200 on the Benchmark XT (CC1 mild, 8 mins primary antibody and ultraview DAB detection).
Mark On Fri, Mar 16, 2012 at 7:34 AM, Rene J Buesa <rjbu...@yahoo.com> wrote: > DAKO monoclonal antibody at 1:1000 for 30 minutes, HIER citrate at pH6, > colon cancer (+) case as control. > René J. > > --- On Fri, 3/16/12, Chakib Boussahmain <chak_...@yahoo.com> wrote: > > > From: Chakib Boussahmain <chak_...@yahoo.com> > Subject: [Histonet] Calponin > To: histonet@lists.utsouthwestern.edu > Date: Friday, March 16, 2012, 9:14 AM > > > Hello Histonet > Does anyone use Calponin stain? if so can you share the protocol with us? > What do you use for epitope retrieval? > Many thanks in advance. > Chakib Boussahmain > Histotechnologist > HTL(ASCP) > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
