Dear histonet! A few hours after submersion of a tissue-block (eg liver) in NBF, the block is cut across and we see the colour-change from brown to grey at the margins until a frontline.
This frontline shows us: a) the distance of penetration of NBF; b) the distance of penetration AND addition of methylol-groups; c) the distance of penetration AND addition of methylol-groups AND (at least beginning) cross-linking. Do you see the issue? I would be happy, if you can help me to find a correct answer. Regards Gudrun Lang Biomed. Analytikerin Histolab Linz, Austria _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet