Cut thin slices of breast (3 mm thick or less), fix them at 45ºC in neutral buffered formalin for AT LEAST 36 hours to assure good fixation = cross-linking. If you do this you will never need to reprocess a block. René J.
--- On Mon, 3/19/12, Mandy O'Connor <ama...@ypii.com> wrote: From: Mandy O'Connor <ama...@ypii.com> Subject: [Histonet] Fixation for breasts To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Date: Monday, March 19, 2012, 11:36 AM Hey fellow histo nuts! Anyone have any good recommendations for fixing breast tissue? We were wondering about an alcoholic formalin but are unsure if it will hinder or mess up the SISH results. We usually end up re-processing the block again just to get a good slide that is semi readable. Thanks for any and all help! Mandy O'Connor HTL(ASCP) Yellowstone Pathology Instit. Billings, MT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
