All,
I have used a new Verhoeff-Van Gieson stain kit (from BBC) for elastic fibers and have run a large number of slides. I am noticing differences in the staining intensities throughout the samples. I see that there are darker sites and lighter sites within the same panelists. This is not due to a treatment effect because these intensity differences are seen in the controls also. I am fairly confident that our tech has sectioned all the samples at 5um and has stained the slides with all the same timings. Does anyone have an explanation as to why this is occurring? The staining intensity differences are mainly in the epidermis but if that is staining darker/lighter my concern is that the elastic fibers in the dermis are doing the same and will skew the results when they are quantified with a spectral camera. Any help will be greatly appreciated. Thank you in advance. Kristopher L. Kalleberg Research Scientist Unilever R&D 40 Merritt Blvd. Trumbull, CT 06611 p 203.381.5765 f 203.381.5476 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet