Yes it should work, placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes as we experienced with similar samples. Hope this helps!
Meghana V. Bapardekar, Ph. D., Dept. of Histopathology, Cleveland Biolabs, 73 High Street, Buffalo, NY - 14203. Phone: 716-849-6810, ext.: 357 Fax: 716-849-6817 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Machin, Leisha E Sent: Friday, April 13, 2012 2:25 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Wrinkles in whole bone mouse tibias Dear Nancy, I had your mouse tibia problem last year; the marrow consistently folded up accordion-style. I believe the problem was solved by placing the block in the water bath (for less than a minute) then into an ice-water slurry for about 3 minutes. Hope this help - best of luck! Leisha Machin This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
