> Message: 1 > Date: Mon, 16 Apr 2012 11:26:47 -0400 > From: "Alonso Martinez-Canabal" <alonso.martinezcana...@utoronto.ca> > Subject: [Histonet] Methyl green going away... > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <000901cd1be5$5c48d470$14da7d50$@utoronto.ca> > Content-Type: text/plain; charset="US-ASCII" > > Hi > > my boss requires me to counterstain some DAB immunohistochemistry with > methyl green. I use for that purpose the methyl green in aquos solution > 0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate > and the methyl green becomes extremely faint with the alcohols. My protocol > requires long periods of alcoholic dehydration very graded. Is there any > idea on how to save the methyl green. Can I use an alcoholic solution? > Thank you very much. > > Alonso
Alonso, Another alternative is; Drying the slide in the oven @~ 60c for 10 min after the methly green counterstain. Madeleine Huey BS, HTL/QIHC (ASCP) Supervisor - Pathology (IPOX & Histology) madeleineh...@elcaminohospital.org _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet