Hi Andrea, I'm not sure about the unfixed tissue, but we got awesome advice from Donna Emge in regards to our wrinkle problems on fixed frozen brains. We would dip a brush in water and run that across the slide (not too much and not too little), then pick up the tissue. My first thought was, "Water in the cryo?", but it really worked. Good luck!
Bobbie -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Emily Sours Sent: Tuesday, May 01, 2012 1:12 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Frozen brains on cryostat If they haven't been fixed, good luck! I've never been able to section unfixed tissue. Then again, I work with embryonic tissue only. Have you tried sectioning at a smaller section size? Emily The whole point of this country is if you want to eat garbage, balloon up to 600 pounds and die of a heart attack at 43, you can! You are free to do so. To me, that’s beautiful. --Ron Swanson On Tue, May 1, 2012 at 12:53 PM, Andrea Ferullo (non-Celgene) < aferu...@celgene.com> wrote: > Hello everyone, > > I recently received rat brain samples that were frozen in liquid nitrogen > and embedded in OCT. I sectioned them on the cryostat and they are coming > out very wrinkled, no matter what technique I use to pick them up. I would > appreciate any tips/tricks that anyone has to offer. Forebrain sections > are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. > Thanks and I look forward to your advice. > > Andrea > > ********************************************************* > THIS ELECTRONIC MAIL MESSAGE AND ANY ATTACHMENT IS > CONFIDENTIAL AND MAY CONTAIN LEGALLY PRIVILEGED > INFORMATION INTENDED ONLY FOR THE USE OF THE INDIVIDUAL > OR INDIVIDUALS NAMED ABOVE. > If the reader is not the intended recipient, or the > employee or agent responsible to deliver it to the > intended recipient, you are hereby notified that any > dissemination, distribution or copying of this > communication is strictly prohibited. If you have > received this communication in error, please reply to the > sender to notify us of the error and delete the original > message. Thank You. > ********************************************************* > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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