I cut mouse brains frozen in OCT with very little problem. I bring the
Cyrostat temp up to between -15 to -13. Pick them up with a warm slide and
seldom have a wrinkle. Brains are unfixed placed in OCT and frozen with liquid
nitrogen.
Donna Reynolds, Chief Histology Lab (IHC)
U.T, M.D. Anderson Cancer Center
Houston, Texas
713-792-8106
Message: 11
Date: Tue, 1 May 2012 12:53:13 -0400
From: "Andrea Ferullo (non-Celgene)" <aferu...@celgene.com>
Subject: [Histonet] Frozen brains on cryostat
To: "Histonet@lists.utsouthwestern.edu"
<Histonet@lists.utsouthwestern.edu>
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<24701b0ef4127f4fa262751aa9674eea029fe97ed...@sumexprdmb03.celgene.com>
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Hello everyone,
I recently received rat brain samples that were frozen in liquid nitrogen and
embedded in OCT. I sectioned them on the cryostat and they are coming out very
wrinkled, no matter what technique I use to pick them up. I would appreciate
any tips/tricks that anyone has to offer. Forebrain sections are ok, but mid,
hind-brain, and cerebellum are giving me a very hard time. Thanks and I look
forward to your advice.
Andrea
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