I cut mouse brains frozen in OCT with very little problem. I bring the Cyrostat temp up to between -15 to -13. Pick them up with a warm slide and seldom have a wrinkle. Brains are unfixed placed in OCT and frozen with liquid nitrogen. Donna Reynolds, Chief Histology Lab (IHC) U.T, M.D. Anderson Cancer Center Houston, Texas 713-792-8106
Message: 11 Date: Tue, 1 May 2012 12:53:13 -0400 From: "Andrea Ferullo (non-Celgene)" <aferu...@celgene.com> Subject: [Histonet] Frozen brains on cryostat To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <24701b0ef4127f4fa262751aa9674eea029fe97ed...@sumexprdmb03.celgene.com> Content-Type: text/plain; charset="us-ascii" Hello everyone, I recently received rat brain samples that were frozen in liquid nitrogen and embedded in OCT. I sectioned them on the cryostat and they are coming out very wrinkled, no matter what technique I use to pick them up. I would appreciate any tips/tricks that anyone has to offer. Forebrain sections are ok, but mid, hind-brain, and cerebellum are giving me a very hard time. Thanks and I look forward to your advice. Andrea ************ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet