Hi John, In the past, I used Neutrophil Elastase from Dako on monkey FFPE tissue; no pretreatment needed; dilution with lot used = 1:50. Worked great. Jan Shivers UMN Vet Diag Lab On Sat, May 5, 2012 at 9:59 AM, John Shelley <jshel...@sanfordburnham.org>wrote:
> Hi All in Histoland, > > Problem solving never ends! I am working up two antibodies for neutrophils > which are NIMP-R14 from Abcam which it is a rat monoclonal and also > Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I > have tried different conditions without any luck and was wondering if > anyone has worked with these antibodies either from Abcam or another > supplier and might have working conditions that have given results in the > past. We are trying this on FFPE sections which the antibodies say they > work on but I am thinking that I may try frozens. I was trying to avoid > this however because I am using these antibodies on mouse WAT. Any help > would be appreciated. Thanks!!! > > Happy Monday morning!!! > Kind Regards! > John J Shelley > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
