After staining dry the sections in an oven at 60ºC for 10 minutes. When completely dried, coverslip as usual. Beware of the mounting medium solvent because it may contain xylene as well. Use one mounting medium without xylene. René J.
--- On Fri, 5/11/12, Andrew Coleman <[email protected]> wrote: From: Andrew Coleman <[email protected]> Subject: [Histonet] Xylene Substitute for Counterstain Clearing To: [email protected] Date: Friday, May 11, 2012, 12:25 PM Hi all, We are performing a neutral red counterstain on tissue sections containing colored polystyrene microspheres. The spheres are inert to alcohol, but are washed out when we clear with xylene to coverslip. The spheres are also supposedly soluble in DMF, acetone, acetonitrile, chloroform and methylene chloride for what its worth. Is it reasonable to coverslip these slides in permanent mount without clearing with xylene after dehydrating the tissue? Or does anyone know of a substitute clearing agent with chemical properties dissimilar enough from xylene that might be worth trying instead? Thanks, Andrew _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
