Hi Ann-
 
May we assume you've confirmed this is happening at embedding and have 
ruled out any floaters happening during cutting?
 
When embedding, keep Kimwipes or other tissues around, keep the wells closed 
and only pull out enough cassettes that you can keeep clean and clear of your 
working area.  Wipe forceps and surfaces between blocks containing fragmented 
or friable tissue, don't put forceps back in the wells without wiping. You can 
stack a few guaze pads on top of the spout to wipe as you replace the forceps 
and change the pads frequently.   Always, always only open one cassettte at a 
time and never leave the station with an open cassette on the station.  Finish 
before standing or recap and replace.  
 
If you are working with currettes, cell blocks, or other cellular, friable 
tissue, open and unwrap on a clean surface (hot or cold - embedding station 
surface or wipe or l'absorb) and don't reuse the surface before wiping or 
replacing.  If you're using knives or scalpels to scrape, make sure the handles 
and connection points aren't harboring residual tissue. Buy those little 
seamless paring knive from the dollar store--they fit in the wells and wipe 
easily.  Use swabs between embedding sessions or between people trading 
places to clean the wells and then clean them again at the end of the embedding 
session.  
 
If you keep molten paraffin in the hold bins, filter or replace frequently and 
do not reuse.  If you keep the hold bins dry, clean routinely (daily)  Clean 
your molten wax chamber periodically to remove contaminants and keep the filter 
from clogging over time. Most embedding station mfc don't condone running 
xylene through the tubes & pumps--clean hot wax will do the job.
 
We always make it the responsibility of each person to clean both at the end of 
embedding AND to clean again before starting to fully assure the wells and 
surfaces were clear and eliminate a possible cross if one person in the chain 
forgets...double system processes like you double check specimen IDs. 
 
You'll go through a whole bunch of kimwipes--but they are much cheaper than 
gauze and SOOO much better than a cross contamination situation.  IF it still 
happens, it's time to track who embeds each block to see if there's a pattern 
by person.  The point is not to write people up but to support developing clean 
habits and to adjust their habits to do it to their best ability.  
 
Wipe wipe wipe wipe wipe!!  Hope this helps!

Cheryl Kerry, HT(ASCP) 
Full Staff Inc. 
Staffing the AP Lab by helping one GREAT Tech at a time.  
281.852.9457 Office
800.756.3309 Phone & Fax 
ad...@fullstaff.org 

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