Hi Gudrun: What you wrote is "news for me". Dichroic filters with specific lambda transmission of good quality can be used for years. The filters I used were Ernst Leitz, from the early 1950's and the transmission intensity remained strong. We checked the life duration of the mercury lamp, but not of the filters. If you are getting weaker signals perhaps is another cause. René J.
________________________________ From: Gudrun Lang <gu.l...@gmx.at> To: histonet@lists.utsouthwestern.edu Sent: Tuesday, June 5, 2012 10:37 AM Subject: [Histonet] Fluorescence-filters Hi! Filters for fluorescencemicroscopy tend to "burn out" after a certain duration of usage. What duration? We have filters for FITC, TRITC, Dapi and a triplefilter. The working-hours are about 150 per year. What do you think? Is it time to change them. I have often bad feedback about weak signals, and I would not be surprised if the microscope is the culprit and not our protocol. Weak signals refer last times to ALK-FISH on lung biopsies. Well fixed but tumourcells mixed within collagenfibers. - and unfortunately unexperienced doctors on reading of this special probe. Hoping for responses Gudrun Lang _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
