True, if you solution is old it may not give the blue color that you want.
Are you doing the overnite procedure? I have found this to be the best. If your 
oven isn't holding at 60º C you could use a waterbath.

Andi G




Andrea Grantham, HT (ASCP)
Senior Research Specialist
University of Arizona
Cellular and Molecular Medicine
Histology Service Laboratory
P.O.Box 245044
Tucson, AZ 85724
º
algra...@email.arizona.edu<mailto:algra...@email.arizona.edu>
Tel: 520.626.4415     Fax: 520.626.2097

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