There is a chart [ http://www.defalcos.com/tutorials/hy.html ] for temperature correction on ethanol concentration. A lot of information can be found on brewery sites. So . . if you require more help, and since its Friday, visit a local brew house to "ask questions".
Tresa :) -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock Sent: Friday, June 29, 2012 8:08 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Hydrometer questions Hi everyone: I wanted to thank everyone who gave me advice regarding my Luxol Fast Blue problem. First, I am going to compare using a very old LFB bottle (from the 1950s) and a new bottle. Then I will stain some test slides for 2 hours and 24 hours at 60C. (I am having my oven checked this morning by facilities). To find out how much water is really in my 95% ethanol, I am assuming that I need a hydrometer. (I will also be using it to measure the isopropanol concentrations on my tissue processor). Is there a type of hydrometer that labs use for these purposes? Where do you buy yours from? Can the alcohol/water concentrations be read right off of the hydrometer, like temperature is from a thermometer, or do you need to use an equation. I have never used a hydrometer before. Thank you, Tim Wheelock Harvard Brain Bank McLean Hospital Belmont, MA 617-855-3592 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet