I have used Vector's ImmPress and ABC secondary antibody systems. I like ImmPress better: it has fewer steps, it gives me less background, and the reagents last longer. Vector's Nova Red has given me better results than any other stain that I have tried. -Allen A. Smith Barry University School of Podiatric Medicine.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Wednesday, June 27, 2012 1:38 PM To: 'Lewis, Patrick'; 'Histonet@lists.utsouthwestern.edu' Subject: RE: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates We use Jackson Immunoresearch for our secondaries and the Biocare Alk. Phosph. Kit or the DAKO DAB plus kit. Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lewis, Patrick Sent: Wednesday, June 27, 2012 9:45 AM To: 'Histonet@lists.utsouthwestern.edu' Subject: [Histonet] Recommendations for HRP anti Rabbit and mouse and AEC substrates Hi everyone, I have been using DAKO Kits for HRP anti rabbit and HRP anti Mouse and AEC substrates. I love DAKO, but their kits and reagents are expensive. Can anyone offer an alternative for HRP labeled anti (Rabbit/Mouse) antibodies and AEC substrate kits. I do all my IHC manually by hand. I am doing my IHCs primarily on FFPE slides of various tissues, some of which are large enough that they take up a lot of the slide, and hence need more drops to get complete coverage. Thanks Patrick. PS: I am considering Vector Labs, and I am also looking at Biocompare to seek out more vendors. Has anyone tried Spring Bioscience's products? CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information protected by law. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet