dilute antibody with %1 BSA.. and use %1 BSA for serum blocking... On Wed, Jul 4, 2012 at 10:59 PM, Mehmet Fatih BOZKURT <fbozk...@gmail.com>wrote:
> dilute antibody with %1 BSA.. and use %1 BSA for serum blocking... > > On Wed, Jul 4, 2012 at 10:21 PM, E Wolfe <ewolfe...@gmail.com> wrote: > >> Hello Histonet - >> >> Has anyone had any success staining with PCNA sc-7907 on frozen, >> paraformaldehyde-fixed tissue? I am experiencing a lot of background, and >> so far diluting down to 1:5000 hasn't helped at all. Any advice would be >> very much appreciated! >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > -- > Mehmet Fatih BOZKURT, DVM, PhD > Afyon Kocatepe University > Faculty of Veterinary Medicine > Department of Pathology > 03030, ANS Campus > Afyonkarahisar-TURKEY > Tel: +902722281312-173/237 > > -- Mehmet Fatih BOZKURT, DVM, PhD Afyon Kocatepe University Faculty of Veterinary Medicine Department of Pathology 03030, ANS Campus Afyonkarahisar-TURKEY Tel: +902722281312-173/237 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
