Hi Kim, I use Sigma V4630 in mouse tissue, but it is sold as an anti-human antibody (but reactive to many species). I use it at a dilution of 1:2500 with a PerkinElmer Cy3 TSA kit, with a Jackson HRP-conjugated secondary. Oh, before I forget, standard AR with citrate, pH 6.
Hope this helps. Jonathan --- Jonathan Cremer Laboratory Technician TARGID - KU Leuven ________________________________________ Van: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] namens Kim Merriam [kmerriam2...@yahoo.com] Verzonden: dinsdag 10 juli 2012 17:48 To: Histonet Onderwerp: [Histonet] human vimentin in mouse tissue Greetings! I want to stain for human vimentin in mouse tissues (human fibroblasts). I many years ago, I used a biotinylated V9 clone for just this purpose and it worked great. I tried the biotiylated V9 clone from abcam, but I am not getting any staining at all. Any suggestions? Kim Kim Merriam, MA, HT(ASCP)QIHC Cambridge, MA _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
